(Histone-H3)-lysine-36 demethylase (EC 1.14.11.27, JHDM1A, JmjC domain-containing histone demethylase 1A, H3-K36-specific demethylase, histone-lysine (H3-K36) demethylase, histone demethylase, protein-6-N,6-N-dimethyl-L-lysine,2-oxoglutarate:oxygen oxidoreductase) is an enzyme with systematic name protein-N6,N6-dimethyl-L-lysine,2-oxoglutarate:oxygen oxidoreductase.^[1] This enzyme catalyses the following chemical reaction

protein N6,N6-dimethyl-L-lysine + 2 2-oxoglutarate + 2 O₂ ${\displaystyle \rightleftharpoons }$ protein L-lysine + 2 succinate + 2 formaldehyde + 2 CO₂ (overall reaction)

(1a) protein N6,N6-dimethyl-L-lysine + 2-oxoglutarate + O₂ ${\displaystyle \rightleftharpoons }$ protein N6-methyl-L-lysine + succinate + formaldehyde + CO₂

(1b) protein N6-methyl-L-lysine + 2-oxoglutarate + O2 ${\displaystyle \rightleftharpoons }$ protein L-lysine + succinate + formaldehyde + CO₂

(Histone-H3)-lysine-36 demethylase contains iron(II).