727 may refer to:

• Boeing 727, an airliner
• AD 727, a year
• 727 BC, a year
• 727 (number), a number
• "727", a song by The Box Tops from the album Cry Like a Baby
• 7/27, a 2016 album by Fifth Harmony
• Area code 727, for telephones in Pinellas County, Florida
• USS Michigan (SSBN-727), a U.S. ballistic missile submarine
• Jet 727, a bus service in Aberdeen, Scotland