The Ernest F. Hollings ACE Basin National Wildlife Refuge is a 11,815-acre (47.81 km²) portion of the larger ACE Basin area, and the only portions that are federally protected. The wildlife refuge is divided into two units: the Edisto River unit and the Combahee River unit.

Ernest F. Hollings ACE Basin National Wildlife Refuge
IUCN category IV (habitat/species management area)
Rice trunk at ACE Basin NWR
Map of the United States Show map of the United States ACE Basin National Wildlife Refuge (South Carolina) Show map of South Carolina
Location	Beaufort, Charleston, Colleton, Hampton counties, South Carolina, United States
Nearest city	Beaufort, South Carolina
Coordinates	32°30′30″N 80°28′00″W / 32.50833°N 80.46667°W / 32.50833; -80.46667
Area	11,815 acres (47.81 km2)
Established	1990
Governing body	U.S. Fish and Wildlife Service
Website	Ernest F. Hollings ACE Basin National Wildlife Refuge

The following threatened or endangered species are present in the refuge: wood stork