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Acid fuchsin

Summary

Acid fuchsin or fuchsine acid, (also called Acid Violet 19[1] and C.I. 42685[1]) is an acidic magenta dye with the chemical formula C20H17N3Na2O9S3. It is a sodium sulfonate derivative of fuchsine. Acid fuchsin has wide use in histology,[1] and is one of the dyes used in Masson's trichrome stain.[2] This method is commonly used to stain cytoplasm and nuclei of tissue sections in the histology laboratory in order to distinguish muscle from collagen. The muscle stains red with the acid fuchsin, and the collagen is stained green or blue with Light Green SF yellowish or methyl blue. It can also be used to identify growing bacteria.[3]

Acid fuchsin
Names
IUPAC name
Disodium 2-amino-5-[(Z)-(4-amino-3-sulfonatophenyl)(4-iminio-3-sulfonato-2,5-cyclohexadien-1-ylidene)methyl]-3-methylbenzenesulfonate
Identifiers
  • 3244-88-0 checkY
3D model (JSmol)
  • Interactive image
ChEBI
  • CHEBI:87052 checkY
ChemSpider
  • 16736101 checkY
ECHA InfoCard 100.019.833 Edit this at Wikidata
EC Number
  • 221-816-5
  • 5464362
UNII
  • 8RA6L21QTM checkY
  • DTXSID8044418 Edit this at Wikidata
  • InChI=1S/C20H19N3O9S3.2Na/c1-10-6-13(9-18(20(10)23)35(30,31)32)19(11-2-4-14(21)16(7-11)33(24,25)26)12-3-5-15(22)17(8-12)34(27,28)29;;/h2-9,21H,22-23H2,1H3,(H,24,25,26)(H,27,28,29)(H,30,31,32);;/q;2*+1/p-2/b19-11-,21-14?;; checkY
    Key: RZUBARUFLYGOGC-MTHOTQAESA-L checkY
  • InChI=1/C20H19N3O9S3.2Na/c1-10-6-13(9-18(20(10)23)35(30,31)32)19(11-2-4-14(21)16(7-11)33(24,25)26)12-3-5-15(22)17(8-12)34(27,28)29;;/h2-9,21H,22-23H2,1H3,(H,24,25,26)(H,27,28,29)(H,30,31,32);;/q;2*+1/p-2/b19-11-,21-14?;;
    Key: RZUBARUFLYGOGC-LOQRZINWBG
  • CC1=CC(=CC(=C1N)S(=O)(=O)[O-])/C(=C\2/C=CC(=[NH2+])C(=C2)S(=O)(=O)[O-])/C3=CC(=C(C=C3)N)S(=O)(=O)[O-].[Na+].[Na+]
Properties
C20H17N3Na2O9S3
Molar mass 585.53 g·mol−1
Hazards
GHS labelling:
GHS07: Exclamation mark
Warning
H315, H319, H335
P261, P264, P271, P280, P302+P352, P304+P340, P305+P351+P338, P312, P321, P332+P313, P337+P313, P362, P403+P233, P405, P501
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Infobox references

See also edit

References edit

  1. ^ a b c Lillie, Ralph Dougall (1977). H. J. Conn's Biological stains (9th ed.). Baltimore: Williams & Wilkins. p. 692.
  2. ^ Jocelyn H. Bruce-Gregorios, M.D.: Histopathologic Techniques, JMC Press Inc., Quezon City, Philippines, 1974. ISBN 971-11-0853-4
  3. ^ Holman, W. L (1914). "The use of Decolorized Acid Fuchsin as an Acid Indicator in Carbohydrate Fermentation Tests with some Remarks on Acid Production by Bacteria". Journal of Infectious Diseases. 15: 227–233. doi:10.1093/infdis/15.1.227.