Aloe khamiesensis (also called Khamiesberg aloe or tweederly in Afrikaans) is a species of plant in the genus Aloe.

Aloe khamiesensis
Conservation status
Data Deficient  (IUCN 2.3)[1]
Scientific classification
Kingdom:	Plantae
Clade:	Tracheophytes
Clade:	Angiosperms
Clade:	Monocots
Order:	Asparagales
Family:	Asphodelaceae
Subfamily:	Asphodeloideae
Genus:	Aloe
Species:	A. khamiesensis
Binomial name
Aloe khamiesensis Pillans

It is endemic to South Africa, where it occurs in the dry, western interior regions of Namaqualand and Bushmanland. It is closely related to Aloe microstigma which occurs in and around the Little Karoo to the south, and to Aloe microstigma subsp. framesii which occurs to the west along the Namaqualand coast.^{[2] [3]}