In enzymology, an aspartate N-acetyltransferase (EC 2.3.1.17) is an enzyme that catalyzes the chemical reaction

acetyl-CoA + L-aspartate ${\displaystyle \rightleftharpoons }$ CoA + N-acetyl-L-aspartate

Thus, the two substrates of this enzyme are acetyl-CoA and L-aspartate, whereas its two products are CoA and N-acetyl-L-aspartate.

This enzyme belongs to the family of transferases, specifically those acyltransferases transferring groups other than aminoacyl groups. The systematic name of this enzyme class is acetyl-CoA:L-aspartate N-acetyltransferase. Other names in common use include aspartate acetyltransferase, and L-aspartate N-acetyltransferase.