Born in Berlin, Bekker completed his classical education at the University of Halle under Friedrich August Wolf, who considered him as his most promising pupil. In 1810 he was appointed professor of philosophy in the University of Berlin. For several years, between 1810 and 1821, he travelled in France, Italy, England and parts of Germany, examining classical manuscripts and gathering materials for his great editorial labours. 
Some of the fruits of his researches were published in the Anecdota Graeca (3 vols, 1814–1821), but the major results are to be found in the enormous array of classical authors edited by him. Anything like a complete list of his works would occupy too much space, but it may be said that his industry extended to nearly the whole of Greek literature with the exception of the tragedians and lyric poets. His best known editions are those of Plato (1816–1823), Oratores Attici (1823–1824), Aristotle (1831–1836), Aristophanes (1829), and twenty-five volumes of the Corpus Scriptorum Historiae Byzantinae. The only Latin authors edited by him were Livy (1829–1830) and Tacitus (1831).
Bekker confined himself entirely to manuscript investigations and textual criticism; he contributed little to the extension of other types of scholarship. Bekker numbers have become the standard way of referring to the works of Aristotle and the Corpus Aristotelicum. He was elected a Foreign Honorary Member of the American Academy of Arts and Sciences in 1861. He died in Berlin aged 86.
- Chisholm 1911, p. 661.
- Vol. 1. Lexica Segueriana 1814 (e.g. Δικῶν ὀνόματα, pp. 181-194; Λέξεις ῥητορικαί, pp. 195-318 etc) – v. 2. Apollonii Alexandrini de coniunctionibus (p. 477) et de adverbiis (p. 527) libri. Dionysii Thracis Grammatica (p. 627). Choerobosci, Diomedis, Melampodis, Porphyrii, Stephani in eam scholia (pp. 645–927). Berolini: apud G. Reimerum 1816 – v. 3. Theodosii canones (p. 975). Editoris annotatio critica (p. 1065). Indices (pp. 1299–1466). Berolini: Typis et impensis G. Reimeri 1821.
- "Book of Members, 1780-2010: Chapter B" (PDF). American Academy of Arts and Sciences. Retrieved 30 May 2011.
- This article incorporates text from a publication now in the public domain: Chisholm, Hugh, ed. (1911). "Bekker, August Immanuel". Encyclopædia Britannica. 3 (11th ed.). Cambridge University Press. p. 661. Endnotes:
- Sauppe, Zur Erinnerung an Meineke und Bekker (1872);
- Haupt, “Gedächtnisrede auf Meineke und Bekker,” in his Opuscula, iii.;
- E. I. Bekker, “Zur Erinnerung an meinen Vater,” in the Preussisches Jahrbuch, xxix.
- Apollonii Dyscoli de Pronomine liber, ed. I. Bekker, Berolini 1813.
- Apollonii Alexandrini de Constructione Orationis libri quatuor ex rec. I. Bekkeri, Berolini 1817.
- Aristotelis Opera edidit Academia Regia Borussica, Berlin, 1831–1870. (5 volumes).