Carbamoyl phosphate synthetase (glutamine-hydrolysing) (EC 6.3.5.5) is an enzyme that catalyzes the reactions that produce carbamoyl phosphate in the cytosol (as opposed to type I, which functions in the mitochondria). Its systemic name is hydrogen-carbonate:L-glutamine amido-ligase (ADP-forming, carbamate-phosphorylating).[1][2][3][4][5][6][7][8]
Carbamoyl-phosphate synthetase (glutamine-hydrolysing) | |||||||||
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Identifiers | |||||||||
EC no. | 6.3.5.5 | ||||||||
CAS no. | 37233-48-0 | ||||||||
Databases | |||||||||
IntEnz | IntEnz view | ||||||||
BRENDA | BRENDA entry | ||||||||
ExPASy | NiceZyme view | ||||||||
KEGG | KEGG entry | ||||||||
MetaCyc | metabolic pathway | ||||||||
PRIAM | profile | ||||||||
PDB structures | RCSB PDB PDBe PDBsum | ||||||||
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carbamoyl-phosphate synthetase 1, aspartate transcarbamylase, and dihydroorotase | |||||||
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Identifiers | |||||||
Symbol | CAD | ||||||
NCBI gene | 790 | ||||||
HGNC | 1424 | ||||||
OMIM | 114010 | ||||||
RefSeq | NM_004341 | ||||||
UniProt | P27708 | ||||||
Other data | |||||||
Locus | Chr. 2 p21 | ||||||
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In pyrimidine biosynthesis, it serves as the rate-limiting enzyme and catalyzes the following reaction:
It is activated by ATP and PRPP[9] and it is inhibited by UTP (Uridine triphosphate)[10] Neither CPSI nor CPSII require biotin as a coenzyme, as seen with most carboxylation reactions.
It is one of the three enzyme functions coded by the CAD gene. It is classified under EC 6.3.5.5.