The following television stations operate on virtual channel 27 in the United States:^[1]

• K12XK-D in Boulder, Colorado
• K12XQ-D in Monroe, Louisiana
• K14NJ-D in Hot Springs, Montana
• K18GG-D in Mina/Luning, Nevada
• K20JX-D in Sacramento, California
• K25OB-D in San Antonio, Texas
• K26CI-D in Cortez, etc., Colorado
• K26JC-D in Walker Lake, Nevada
• K26JI-D in Sibley, Iowa
• K27AE-D in Victorville, etc., California
• K27DO-D in Bend, etc., Oregon
• K27JP-D in Little Rock, Arkansas
• K27MQ-D in St. George, Utah
• K27OY-D in Memphis, Tennessee
• K27PC-D in Yuma, Arizona
• K27PE-D in Gustine, California
• K32EY-D in Dove Creek, etc., Colorado
• K33GZ-D in Hawthorne, Nevada
• K33PV-D in Rock Rapids, Iowa
• KAOB-LD in Beaumont, Texas
• KBAX-LD in Twin Falls, Idaho
• KBKI-LD in Boise, Idaho
• KCWS-LD in Sioux Falls, South Dakota
• KCWV in Duluth, Minnesota
• KDFI in Dallas, Texas
• KDKJ-LD in Tyler, Texas
• KELV-LD in Las Vegas, Nevada
• KFDY-LD in Lincoln, Nebraska
• KGHB-CD in Pueblo, etc., Colorado
• KGJT-CD in Grand Junction, Colorado
• KHGI-CD in North Platte, Nebraska
• KJKZ-LD in Fresno, California
• KKEL in Ely, Nevada
• KLDO-TV in Laredo, Texas
• KLWY in Cheyenne, Wyoming
• KMBY-LD in Monterey, California
• KNGF in Grand Forks, North Dakota
• KNXG-LD in College Station, Texas
• KOHA-LD in Omaha, Nebraska
• KOZL-TV in Springfield, Missouri
• KPCD-LD in San Fernando, California
• KREN-TV in Reno, Nevada
• KRPV-DT in Roswell, New Mexico
• KSCD-LD in Hemet, California
• KSFV-CD in Los Angeles, California
• KSIN-TV in Sioux City, Iowa
• KSKC-CD in Pablo/Ronan, Montana
• KSLM-LD in Dallas, Oregon
• KSNT in Topeka, Kansas
• KUAS-TV in Tucson, Arizona
• KUCO-LD in Chico, California
• KUKR-LD in Santa Rosa, California
• KWBH-LD in Rapid City, South Dakota
• KWTC-LD in Kerrville, Texas
• KYPO-LD in Tacna, Arizona
• W15DC-D in Florence, South Carolina
• W15ES-D in Myrtle Beach, South Carolina
• W26FE-D in Montgomery, Alabama
• W27AU-D in Wausau, Wisconsin
• W27DK-D in Columbus, Georgia
• W27DP-D in New Bern, North Carolina
• W27DQ-D in Elmhurst, Michigan
• W27EO-D in Panama City, Florida
• W27EP-D in Destin, Florida
• W27EQ-D in Peoria, Illinois
• W27ET-D in Maple Valley, Michigan
• WBGU-TV in Bowling Green, Ohio
• WCCU in Urbana, Illinois
• WCMV in Cadillac, Michigan
• WCQT-LD in Cullman, Alabama
• WFXR in Roanoke, Virginia
• WGEI-LD in Enterprise, Alabama
• WGNT in Portsmouth, Virginia
• WGZT-LD in Key West, Florida
• WHJC-LP in Williamson, West Virginia
• WHTM-TV in Harrisburg, Pennsylvania
• WIRP-LD in Raleigh, North Carolina
• WKBN-TV in Youngstown, Ohio
• WKOW in Madison, Wisconsin
• WKYT-TV in Lexington, Kentucky
• WLNM-LD in Lansing, Michigan
• WLOV-TV in West Point, Mississippi
• WLPB-TV in Baton Rouge, Louisiana
• WNAL-LD in Scottsboro, Alabama
• WNYK-LD in Teaneck, New Jersey
• WOCD-LD in Ocala, Florida
• WQEC in Quincy, Illinois
• WRDQ in Orlando, Florida
• WRJA-TV in Sumter, South Carolina
• WSOT-LD in Marion, Indiana
• WTCT in Marion, Illinois
• WTNB-CD in Cleveland, Tennessee
• WTXL-TV in Tallahassee, Florida
• WUNW in Canton, North Carolina
• WUTF-TV in Worcester, Massachusetts
• WWAX-LD in Hartford, Connecticut
• WWRJ-LD in Jacksonville, Florida
• WXSG-LD in Springfield, Illinois
• WYJJ-LD in Jackson, Tennessee

The following stations, which are no longer licensed, formerly operated on virtual channel 27 in the United States:

• K11XT-D in Mariposa, California
• K20CP-D in Elmo, Montana
• K27EC-D in Lake Havasu City, Arizona
• K27MG-D in Columbia, Missouri
• KCPM in Grand Forks, North Dakota
• KEXT-CD in San Jose, California
• KILW-LD in Rochester, Minnesota
• KNYS-LD in Natchitoches, Louisiana
• KULG-LD in Springfield, Missouri
• W27DH-D in Evansville, Indiana
• W27DV-D in Bluffton–Hilton Head, Georgia
• WDYH-LD in Augusta, Georgia
• WUDI-LD in Myrtle Beach, South Carolina