Cyberknife may refer to:

• Cyberknife (horse), a Thoroughbred race horse, winner of the 2022 Arkansas Derby
• Cyberknife (device), is a radiation therapy device manufactured by Accuray Incorporated
• Oklahoma CyberKnife, is a cancer treatment center based in Oklahoma
• Reno CyberKnife, is a cancer treatment center based in Reno, Nevada