DCTN1 Knowpia

DCTN1
Available structures
PDB	Ortholog search: PDBe RCSB
List of PDB id codes
	1TXQ, 2COY, 2HKN, 2HKQ, 2HL3, 2HL5, 2HQH, 3E2U, 3TQ7
Identifiers
Aliases	DCTN1, DAP-150, DP-150, P135, dynactin subunit 1
External IDs	OMIM: 601143 MGI: 107745 HomoloGene: 3011 GeneCards: DCTN1
Gene location (Human)
Chr.	Chromosome 2 (human)
End	74,392,087 bp
Gene location (Mouse)
Chr.	Chromosome 6 (mouse)
End	83,177,099 bp
RNA expression pattern
	Top expressed in
	prefrontal cortex; ; nucleus accumbens; ; amygdala; ; putamen; ; caudate nucleus; ; anterior pituitary; ; Brodmann area 9; ; hypothalamus; ; sural nerve; ; left ventricle;
	Top expressed in
	entorhinal cortex; ; superior frontal gyrus; ; cerebellar cortex; ; lip; ; hippocampus proper; ; superior colliculus; ; esophagus; ; dorsomedial hypothalamic nucleus; ; medulla oblongata; ; primary motor cortex;
	More reference expression data
	; ;
	More reference expression data
Gene ontology
Molecular function	protein binding; cytoskeletal motor activity; microtubule binding; tubulin binding; dynein complex binding; protein kinase binding; tau protein binding;
Cellular component	cytoplasm; centrosome; membrane; dynactin complex; retromer complex; cell leading edge; dynein complex; microtubule; cytoskeleton; kinetochore; microtubule organizing center; nucleus; nuclear envelope; centriole; spindle; cytosol; actin cytoskeleton; microtubule plus-end; spindle pole; cell cortex region; cell cortex; microtubule associated complex; axon; neuron projection; neuronal cell body; intermediate filament cytoskeleton; centriolar subdistal appendage;
Biological process	antigen processing and presentation of exogenous peptide antigen via MHC class II; nervous system development; endoplasmic reticulum to Golgi vesicle-mediated transport; transport along microtubule; IRE1-mediated unfolded protein response; retrograde transport, endosome to Golgi; G2/M transition of mitotic cell cycle; melanosome transport; centriole-centriole cohesion; positive regulation of microtubule polymerization; microtubule anchoring at centrosome; nuclear membrane disassembly; positive regulation of microtubule nucleation; ciliary basal body-plasma membrane docking; non-motile cilium assembly; mitotic cell cycle; establishment of mitotic spindle orientation; regulation of mitotic spindle organization; cell cycle; cell division; regulation of G2/M transition of mitotic cell cycle; transport; neuromuscular junction development; ventral spinal cord development; neuromuscular process; motor behavior; neuron cellular homeostasis; axonal transport; maintenance of synapse structure; positive regulation of neuromuscular junction development; neuron projection maintenance;
	Sources:Amigo / QuickGO
Orthologs
	1639
	13191
	ENSG00000204843
	ENSMUSG00000031865
	Q14203
	O08788
NM_001135040; NM_001135041; NM_001190836; NM_001190837; NM_004082;
	NM_023019; NM_001378991; NM_001378992
	NM_001198866; NM_001198867; NM_007835; NM_001347310
NP_001128512; NP_001128513; NP_001177765; NP_001177766; NP_004073;
	NP_075408; NP_001365920; NP_001365921
	NP_001185795; NP_001185796; NP_001334239; NP_031861
	Wikidata
View/Edit Human	View/Edit Mouse

Dynactin subunit 1 is a protein that in humans is encoded by the DCTN1 gene.^[5]

Function edit

This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 23 subunits (11 individual proteins ranging in size from 22 to 150 kD).^[6] Dynactin binds to cytoplasmic dynein, dynein cargo adaptors, and microtubules.^[7] It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis.

This subunit is commonly referred to p150-glued.^[5] It is present in two copies per dynactin complex and forms an ≈75 nm long flexible arm that extends from the main body of dynactin.^[6] The p150-glued arm contains binding sites for microtubules,^[8] the microtubule plus tip binding protein EB1,^[9] and the N-terminus of the dynein intermediate chain.^[10]^[11]

Alternative splicing of this gene results in at least 2 functionally distinct isoforms: a ubiquitously expressed one and a brain-specific one. Based on its cytogenetic location, this gene is considered as a candidate gene for limb-girdle muscular dystrophy.^[12]

Interactions edit

DCTN1 has been shown to interact with:

References edit

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000204843 – Ensembl, May 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000031865 – Ensembl, May 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ ^a ^b Holzbaur EL, Hammarback JA, Paschal BM, Kravit NG, Pfister KK, Vallee RB (June 1991). "Homology of a 150K cytoplasmic dynein-associated polypeptide with the Drosophila gene Glued". Nature. 351 (6327): 579–83. Bibcode:1991Natur.351..579H. doi:10.1038/351579a0. PMID 1828535. S2CID 4263574.
^ ^a ^b Urnavicius L, Zhang K, Diamant AG, Motz C, Schlager MA, Yu M, Patel NA, Robinson CV, Carter AP (March 2015). "The structure of the dynactin complex and its interaction with dynein". Science. 347 (6229): 1441–1446. Bibcode:2015Sci...347.1441U. doi:10.1126/science.aaa4080. PMC 4413427. PMID 25814576.
^ Cianfrocco MA, DeSantis ME, Leschziner AE, Reck-Peterson SL (2015). "Mechanism and regulation of cytoplasmic dynein". Annual Review of Cell and Developmental Biology. 31: 83–108. doi:10.1146/annurev-cellbio-100814-125438. PMC 4644480. PMID 26436706.
^ Waterman-Storer CM, Karki S, Holzbaur EL (February 1995). "The p150Glued component of the dynactin complex binds to both microtubules and the actin-related protein centractin (Arp-1)". Proceedings of the National Academy of Sciences of the United States of America. 92 (5): 1634–8. Bibcode:1995PNAS...92.1634W. doi:10.1073/pnas.92.5.1634. PMC 42574. PMID 7878030.
^ Berrueta L, Tirnauer JS, Schuyler SC, Pellman D, Bierer BE (April 1999). "The APC-associated protein EB1 associates with components of the dynactin complex and cytoplasmic dynein intermediate chain". Current Biology. 9 (8): 425–8. Bibcode:1999CBio....9..425B. doi:10.1016/S0960-9822(99)80190-0. PMID 10226031. S2CID 14191776.
^ Karki S, Holzbaur EL (December 1995). "Affinity chromatography demonstrates a direct binding between cytoplasmic dynein and the dynactin complex". The Journal of Biological Chemistry. 270 (48): 28806–11. doi:10.1074/jbc.270.48.28806. PMID 7499404.
^ Vaughan KT, Vallee RB (December 1995). "Cytoplasmic dynein binds dynactin through a direct interaction between the intermediate chains and p150Glued". The Journal of Cell Biology. 131 (6 Pt 1): 1507–16. doi:10.1083/jcb.131.6.1507. PMC 2120689. PMID 8522607.
^ "Entrez Gene: DCTN1 dynactin 1 (p150, glued homolog, Drosophila)".
^ Kim JC, Badano JL, Sibold S, Esmail MA, Hill J, Hoskins BE, Leitch CC, Venner K, Ansley SJ, Ross AJ, Leroux MR, Katsanis N, Beales PL (May 2004). "The Bardet-Biedl protein BBS4 targets cargo to the pericentriolar region and is required for microtubule anchoring and cell cycle progression". Nature Genetics. 36 (5): 462–70. doi:10.1038/ng1352. PMID 15107855.
^ Liu JJ, Ding J, Kowal AS, Nardine T, Allen E, Delcroix JD, Wu C, Mobley W, Fuchs E, Yang Y (October 2003). "BPAG1n4 is essential for retrograde axonal transport in sensory neurons". The Journal of Cell Biology. 163 (2): 223–9. doi:10.1083/jcb.200306075. PMC 2173519. PMID 14581450.
^ Sahni M, Zhou XM, Bakiri L, Schlessinger J, Baron R, Levy JB (December 1996). "Identification of a novel 135-kDa Grb2-binding protein in osteoclasts". The Journal of Biological Chemistry. 271 (51): 33141–7. doi:10.1074/jbc.271.51.33141. PMID 8955163.
^ Short B, Preisinger C, Schaletzky J, Kopajtich R, Barr FA (October 2002). "The Rab6 GTPase regulates recruitment of the dynactin complex to Golgi membranes". Current Biology. 12 (20): 1792–5. Bibcode:2002CBio...12.1792S. doi:10.1016/s0960-9822(02)01221-6. PMID 12401177. S2CID 14031165.

External links edit

GeneReviews/NIH/NCBI/UW entry on Perry syndrome

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000204843 – Ensembl, May 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000031865 – Ensembl, May 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid1828535-5] Holzbaur EL, Hammarback JA, Paschal BM, Kravit NG, Pfister KK, Vallee RB (June 1991). "Homology of a 150K cytoplasmic dynein-associated polypeptide with the Drosophila gene Glued". Nature. 351 (6327): 579–83. Bibcode:1991Natur.351..579H. doi:10.1038/351579a0. PMID 1828535. S2CID 4263574.

[:0-6] Urnavicius L, Zhang K, Diamant AG, Motz C, Schlager MA, Yu M, Patel NA, Robinson CV, Carter AP (March 2015). "The structure of the dynactin complex and its interaction with dynein". Science. 347 (6229): 1441–1446. Bibcode:2015Sci...347.1441U. doi:10.1126/science.aaa4080. PMC 4413427. PMID 25814576.

[7] Cianfrocco MA, DeSantis ME, Leschziner AE, Reck-Peterson SL (2015). "Mechanism and regulation of cytoplasmic dynein". Annual Review of Cell and Developmental Biology. 31: 83–108. doi:10.1146/annurev-cellbio-100814-125438. PMC 4644480. PMID 26436706.

[8] Waterman-Storer CM, Karki S, Holzbaur EL (February 1995). "The p150Glued component of the dynactin complex binds to both microtubules and the actin-related protein centractin (Arp-1)". Proceedings of the National Academy of Sciences of the United States of America. 92 (5): 1634–8. Bibcode:1995PNAS...92.1634W. doi:10.1073/pnas.92.5.1634. PMC 42574. PMID 7878030.

[9] Berrueta L, Tirnauer JS, Schuyler SC, Pellman D, Bierer BE (April 1999). "The APC-associated protein EB1 associates with components of the dynactin complex and cytoplasmic dynein intermediate chain". Current Biology. 9 (8): 425–8. Bibcode:1999CBio....9..425B. doi:10.1016/S0960-9822(99)80190-0. PMID 10226031. S2CID 14191776.

[10] Karki S, Holzbaur EL (December 1995). "Affinity chromatography demonstrates a direct binding between cytoplasmic dynein and the dynactin complex". The Journal of Biological Chemistry. 270 (48): 28806–11. doi:10.1074/jbc.270.48.28806. PMID 7499404.

[11] Vaughan KT, Vallee RB (December 1995). "Cytoplasmic dynein binds dynactin through a direct interaction between the intermediate chains and p150Glued". The Journal of Cell Biology. 131 (6 Pt 1): 1507–16. doi:10.1083/jcb.131.6.1507. PMC 2120689. PMID 8522607.

[entrez-12] "Entrez Gene: DCTN1 dynactin 1 (p150, glued homolog, Drosophila)".

[pmid15107855-13] Kim JC, Badano JL, Sibold S, Esmail MA, Hill J, Hoskins BE, Leitch CC, Venner K, Ansley SJ, Ross AJ, Leroux MR, Katsanis N, Beales PL (May 2004). "The Bardet-Biedl protein BBS4 targets cargo to the pericentriolar region and is required for microtubule anchoring and cell cycle progression". Nature Genetics. 36 (5): 462–70. doi:10.1038/ng1352. PMID 15107855.

[pmid14581450-14] Liu JJ, Ding J, Kowal AS, Nardine T, Allen E, Delcroix JD, Wu C, Mobley W, Fuchs E, Yang Y (October 2003). "BPAG1n4 is essential for retrograde axonal transport in sensory neurons". The Journal of Cell Biology. 163 (2): 223–9. doi:10.1083/jcb.200306075. PMC 2173519. PMID 14581450.

[pmid8955163-15] Sahni M, Zhou XM, Bakiri L, Schlessinger J, Baron R, Levy JB (December 1996). "Identification of a novel 135-kDa Grb2-binding protein in osteoclasts". The Journal of Biological Chemistry. 271 (51): 33141–7. doi:10.1074/jbc.271.51.33141. PMID 8955163.

[pmid12401177-16] Short B, Preisinger C, Schaletzky J, Kopajtich R, Barr FA (October 2002). "The Rab6 GTPase regulates recruitment of the dynactin complex to Golgi membranes". Current Biology. 12 (20): 1792–5. Bibcode:2002CBio...12.1792S. doi:10.1016/s0960-9822(02)01221-6. PMID 12401177. S2CID 14031165.

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Summary

Function edit

Interactions edit

References edit

Further reading edit

External links edit