The series follows nine ladettes (i.e. loud, foul-mouthed, uncultured and unpleasant women, who resort to drinking, smoking and often sexually promiscuous behaviour), who are given an old-fashioned six-week course in learning how to behave like a real lady. In the first season, they were sent to Huis 't Velde, an International Butler Academy in Warnsveld, Gelderland. In the second season they were sent to Nieuw Rande, in Diepenveen, Overijssel. Nieuw Rande was specially reopened to try to change the ladettes' habits.

Whilst in Nieuw Rande they are required to wear the special uniform: a tweed suit including a turquoise jacket, white blouse, and turquoise skirt, high-heeled shoes and a pearl necklace. They are taught under the supervision and guidance of five instructors and are given various tasks in deportment, elocution, etiquette and cookery. However, if they can't live up to the standards of acting ladylike, they are expelled. Each episode sees one ladette (sometimes two) expelled till only three are left at the end of the season. Then the teachers must decide upon one winner, depending on who they think has improved the most and how the ladettes performed in the final challenge.

• Official site
• Dames in de dop 2008 compilatie aflevering 3 on YouTube (in Dutch)