A genome sequence is the complete list of the nucleotides (A, C, G, and T for DNA genomes) that make up all the chromosomes of an individual or a species. Within a species, the vast majority of nucleotides are identical between individuals, but sequencing multiple individuals is necessary to understand the genetic diversity.
Part of DNA sequence - prototypification of complete genome of virus
The development of new technologies has made genome sequencing dramatically cheaper and easier, and the number of complete genome sequences is growing rapidly. The US National Institutes of Health maintains one of several comprehensive databases of genomic information. Among the thousands of completed genome sequencing projects include those for rice, a mouse, the plant Arabidopsis thaliana, the puffer fish, and the bacteria E. coli. In December 2013, scientists first sequenced the entire genome of a Neanderthal, an extinct species of humans. The genome was extracted from the toe bone of a 130,000-year-old Neanderthal found in a Siberian cave.
Whereas a genome sequence lists the order of every DNA base in a genome, a genome map identifies the landmarks. A genome map is less detailed than a genome sequence and aids in navigating around the genome. The Human Genome Project was organized to map and to sequence the human genome. A fundamental step in the project was the release of a detailed genomic map by Jean Weissenbach and his team at the Genoscope in Paris.
Viral genomes can be composed of either RNA or DNA. The genomes of RNA viruses can be either single-stranded or double-stranded RNA, and may contain one or more separate RNA molecules (segments: monopartit or multipartit genome). DNA viruses can have either single-stranded or double-stranded genomes. Most DNA virus genomes are composed of a single, linear molecule of DNA, but some are made up of a circular DNA molecule.
Prokaryotes and eukaryotes have DNA genomes. Archaea have a single circular chromosome. Most bacteria also have a single circular chromosome; however, some bacterial species have linear chromosomes or multiple chromosomes. If the DNA is replicated faster than the bacterial cells divide, multiple copies of the chromosome can be present in a single cell, and if the cells divide faster than the DNA can be replicated, multiple replication of the chromosome is initiated before the division occurs, allowing daughter cells to inherit complete genomes and already partially replicated chromosomes. Most prokaryotes have very little repetitive DNA in their genomes. However, some symbiotic bacteria (e.g. Serratia symbiotica) have reduced genomes and a high fraction of pseudogenes: only ~40% of their DNA encodes proteins.
Some bacteria have auxiliary genetic material, also part of their genome, which is carried in plasmids. For this, the word genome should not be used as a synonym of chromosome.
Eukaryotic genomes are composed of one or more linear DNA chromosomes. The number of chromosomes varies widely from Jack jumper ants and an asexual nemotode, which each have only one pair, to a fern species that has 720 pairs. A typical human cell has two copies of each of 22 autosomes, one inherited from each parent, plus two sex chromosomes, making it diploid. Gametes, such as ova, sperm, spores, and pollen, are haploid, meaning they carry only one copy of each chromosome.
In addition to the chromosomes in the nucleus, organelles such as the chloroplasts and mitochondria have their own DNA. Mitochondria are sometimes said to have their own genome often referred to as the "mitochondrial genome". The DNA found within the chloroplast may be referred to as the "plastome". Like the bacteria they originated from, mitochondria and chloroplasts have a circular chromosome.
Unlike prokaryotes, eukaryotes have exon-intron organization of protein coding genes and variable amounts of repetitive DNA. In mammals and plants, the majority of the genome is composed of repetitive DNA.
DNA sequences that carry the instructions to make proteins are coding sequences. The proportion of the genome occupied by coding sequences varies widely. A larger genome does not necessarily contain more genes, and the proportion of non-repetitive DNA decreases along with increasing genome size in complex eukaryotes.
Simple eukaryotes such as C. elegans and fruit fly, have more non-repetitive DNA than repetitive DNA, while the genomes of more complex eukaryotes tend to be composed largely of repetitive DNA. In some plants and amphibians, the proportion of repetitive DNA is more than 80%. Similarly, only 2% of the human genome codes for proteins.
Composition of the human genome
Noncoding sequences include introns, sequences for non-coding RNAs, regulatory regions, and repetitive DNA. Noncoding sequences make up 98% of the human genome. There are two categories of repetitive DNA in the genome: tandem repeats and interspersed repeats.
Short, non-coding sequences that are repeated head-to-tail are called tandem repeats. Microsatellites consisting of 2-5 basepair repeats, while minisatellite repeats are 30-35 bp. Tandem repeats make up about 4% of the human genome and 9% of the fruit fly genome. Tandem repeats can be functional. For example, telomeres are composed of the tandem repeat TTAGGG in mammals, and they play an important role in protecting the ends of the chromosome.
In other cases, expansions in the number of tandem repeats in exons or introns can cause disease. For example, the human gene huntingtin typically contains 6–29 tandem repeats of the nucleotides CAG (encoding a polyglutamine tract). An expansion to over 36 repeats results in Huntington's disease, a neurodegenerative disease. Twenty human disorders are known to result from similar tandem repeat expansions in various genes. The mechanism by which proteins with expanded polygulatamine tracts cause death of neurons is not fully understood. One possibility is that the proteins fail to fold properly and avoid degradation, instead accumulating in aggregates that also sequester important transcription factors, thereby altering gene expression.
Tandem repeats are usually caused by slippage during replication, unequal crossing-over and gene conversion.
Transposable elements (TEs) are sequences of DNA with a defined structure that are able to change their location in the genome. TEs are categorized as either class I TEs, which replicate by a copy-and-paste mechanism, or class II TEs, which can be excised from the genome and inserted at a new location.
The movement of TEs is a driving force of genome evolution in eukaryotes because their insertion can disrupt gene functions, homologous recombination between TEs can produce duplications, and TE can shuffle exons and regulatory sequences to new locations.
Long terminal repeats (LTRs) are derived from ancient retroviral infections, so they encode proteins related to retroviral proteins including gag (structural proteins of the virus), pol (reverse transcriptase and integrase), pro (protease), and in some cases env (envelope) genes. These genes are flanked by long repeats at both 5' and 3' ends. It has been reported that LTRs consist of the largest fraction in most plant genome and might account for the huge variation in genome size.
Long interspersed elements (LINEs) encode genes for reverse transcriptase and endonuclease, making them autonomous transposable elements. The human genome has around 500,000 LINEs, taking around 17% of the genome.
Short interspersed elements (SINEs) are usually less than 500 base pairs and are non-autonomous, so they rely on the proteins encoded by LINEs for transposition. The Alu element is the most common SINE found in primates. It is about 350 base pairs and occupies about 11% of the human genome with around 1,500,000 copies.
DNA transposons encode a transposase enzyme between inverted terminal repeats. When expressed, the transposase recognizes the terminal inverted repeats that flank the transposon and catalyzes its excision and reinsertion in a new site. This cut-and-paste mechanism typically reinserts transposons near their original location (within 100kb). DNA transposons are found in bacteria and make up 3% of the human genome and 12% of the genome of the roundworm C. elegans.
Log-log plot of the total number of annotated proteins in genomes submitted to GenBank as a function of genome size.
Genome size is the total number of DNA base pairs in one copy of a haploid genome. In humans, the nuclear genome comprises approximately 3.2 billion nucleotides of DNA, divided into 24 linear molecules, the shortest 50 000 000 nucleotides in length and the longest 260 000 000 nucleotides, each contained in a different chromosome. The genome size is positively correlated with the morphological complexity among prokaryotes and lower eukaryotes; however, after mollusks and all the other higher eukaryotes above, this correlation is no longer effective. This phenomenon also indicates the mighty influence coming from repetitive DNA on the genomes.
Since genomes are very complex, one research strategy is to reduce the number of genes in a genome to the bare minimum and still have the organism in question survive. There is experimental work being done on minimal genomes for single cell organisms as well as minimal genomes for multi-cellular organisms (see Developmental biology). The work is both in vivo and in silico.
Here is a table of some significant or representative genomes. See #See also for lists of sequenced genomes.
All the cells of an organism originate from a single cell, so they are expected to have identical genomes; however, in some cases, differences arise. Both the process of copying DNA during cell division and exposure to environmental mutagens can result in mutations in somatic cells. In some cases, such mutations lead to cancer because they cause cells to divide more quickly and invade surrounding tissues. In certain lymphocytes in the human immune system, V(D)J recombination generates different genomic sequences such that each cell produces a unique antibody or T cell receptors.
During meiosis, diploid cells divide twice to produce haploid germ cells. During this process, recombination results in a reshuffling of the genetic material from homologous chromosomes so each gamete has a unique genome.
Genomes are more than the sum of an organism's genes and have traits that may be measured and studied without reference to the details of any particular genes and their products. Researchers compare traits such as karyotype (chromosome number), genome size, gene order, codon usage bias, and GC-content to determine what mechanisms could have produced the great variety of genomes that exist today (for recent overviews, see Brown 2002; Saccone and Pesole 2003; Benfey and Protopapas 2004; Gibson and Muse 2004; Reese 2004; Gregory 2005).
Duplications play a major role in shaping the genome. Duplication may range from extension of short tandem repeats, to duplication of a cluster of genes, and all the way to duplication of entire chromosomes or even entire genomes. Such duplications are probably fundamental to the creation of genetic novelty.
Horizontal gene transfer is invoked to explain how there is often an extreme similarity between small portions of the genomes of two organisms that are otherwise very distantly related. Horizontal gene transfer seems to be common among many microbes. Also, eukaryotic cells seem to have experienced a transfer of some genetic material from their chloroplast and mitochondrial genomes to their nuclear chromosomes. Recent empirical data suggest an important role of viruses and sub-viral RNA-networks to represent a main driving role to generate genetic novelty and natural genome editing.
Works of science fiction illustrate concerns about the availability of genome sequences.
Michael Crichton's 1990 novel Jurassic Park and the subsequent film tell the story of a billionaire who creates a theme park of cloned dinosaurs on a remote island, with disastrous outcomes. A geneticist extracts dinosaur DNA from the blood of ancient mosquitoes and fills in the gaps with DNA from modern species to create several species of dinosaurs. A chaos theorist is asked to give his expert opinion on the safety of engineering an ecosystem with the dinosaurs, and he repeatedly warns that the outcomes of the project will be unpredictable and ultimately uncontrollable. These warnings about the perils of using genomic information are a major theme of the book.
The 1997 film Gattaca is set in a futurist society where genomes of children are engineered to contain the most ideal combination of their parents' traits, and metrics such as risk of heart disease and predicted life expectancy are documented for each person based on their genome. People conceived outside of the eugenics program, known as "In-Valids" suffer discrimination and are relegated to menial occupations. The protagonist of the film is an In-Valid who works to defy the supposed genetic odds and achieve his dream of working as a space navigator. The film warns against a future where genomic information fuels prejudice and extreme class differences between those who can and can't afford genetically engineered children.
^Brosius, J (2009), "The Fragmented Gene", Annals of the New York Academy of Sciences, 1178 (1): 186–93, Bibcode:2009NYASA1178..186B, doi:10.1111/j.1749-6632.2009.05004.x, PMID19845638
^Ridley M (2006). Genome: the autobiography of a species in 23 chapters(PDF). New York: Harper Perennial. ISBN 978-0-06-019497-0. Archived from the original (PDF) on 24 October 2018. Retrieved 11 May 2016.
^Winkler HL (1920). Verbreitung und Ursache der Parthenogenesis im Pflanzen- und Tierreiche. Jena: Verlag Fischer.
^"definition of Genome in Oxford dictionary". Retrieved 25 March 2014.
^Lederberg J, McCray AT (2001). "'Ome Sweet 'Omics – A Genealogical Treasury of Words" (PDF). The Scientist. 15 (7). Archived from the original (PDF) on 29 September 2006.
^Prüfer K, Racimo F, Patterson N, Jay F, Sankararaman S, Sawyer S, et al. (January 2014). "The complete genome sequence of a Neanderthal from the Altai Mountains". Nature. 505 (7481): 43–49. Bibcode:2014Natur.505...43P. doi:10.1038/nature12886. PMC4031459. PMID24352235.
^Wade N (31 May 2007). "Genome of DNA Pioneer Is Deciphered". The New York Times. Retrieved 2 April 2010.
^"What's a Genome?". Genomenewsnetwork.org. 15 January 2003. Retrieved 27 January 2011.
^NCBI_user_services (29 March 2004). "Mapping Factsheet". Archived from the original on 19 July 2010. Retrieved 27 January 2011.
^Genome Reference Consortium. "Assembling the Genome". Retrieved 23 August 2016.
^Kaplan, Sarah (17 April 2016). "How do your 20,000 genes determine so many wildly different traits? They multitask". The Washington Post. Retrieved 27 August 2016.
^Check Hayden, Erika (2015). "Scientists hope to attract millions to 'DNA.LAND'". Nature. doi:10.1038/nature.2015.18514.
^Zimmer, Carl. "Game of Genomes, Episode 13: Answers and Questions". STAT. Retrieved 27 August 2016.
^Gelderblom, Hans R. (1996). Medical Microbiology (4th ed.). Galveston, TX: The University of Texas Medical Branch at Galveston.
^Samson RY, Bell SD (2014). "Archaeal chromosome biology". Journal of Molecular Microbiology and Biotechnology. 24 (5–6): 420–27. doi:10.1159/000368854. PMC5175462. PMID25732343.
^Chaconas G, Chen CW (2005). "Replication of Linear Bacterial Chromosomes: No Longer Going Around in Circles". The Bacterial Chromosome: 525–540. doi:10.1128/9781555817640.ch29. ISBN 9781555812324.
^ abKoonin EV, Wolf YI (July 2010). "Constraints and plasticity in genome and molecular-phenome evolution". Nature Reviews. Genetics. 11 (7): 487–98. doi:10.1038/nrg2810. PMC3273317. PMID20548290.
^McCutcheon JP, Moran NA (November 2011). "Extreme genome reduction in symbiotic bacteria". Nature Reviews. Microbiology. 10 (1): 13–26. doi:10.1038/nrmicro2670. PMID22064560.
^Land M, Hauser L, Jun SR, Nookaew I, Leuze MR, Ahn TH, Karpinets T, Lund O, Kora G, Wassenaar T, Poudel S, Ussery DW (March 2015). "Insights from 20 years of bacterial genome sequencing". Functional & Integrative Genomics. 15 (2): 141–61. doi:10.1007/s10142-015-0433-4. PMC4361730. PMID25722247.
^"Scientists sequence asexual tiny worm whose lineage stretches back 18 million years". ScienceDaily. Retrieved 7 November 2017.
^Khandelwal S (March 1990). "Chromosome evolution in the genus Ophioglossum L.". Botanical Journal of the Linnean Society. 102 (3): 205–17. doi:10.1111/j.1095-8339.1990.tb01876.x.
^ abcdeLewin B (2004). Genes VIII (8th ed.). Upper Saddle River, NJ: Pearson/Prentice Hall. ISBN 978-0-13-143981-8.
^Naclerio G, Cangiano G, Coulson A, Levitt A, Ruvolo V, La Volpe A (July 1992). "Molecular and genomic organization of clusters of repetitive DNA sequences in Caenorhabditis elegans". Journal of Molecular Biology. 226 (1): 159–68. doi:10.1016/0022-2836(92)90131-3. PMID1619649.
^Stojanovic N, ed. (2007). Computational genomics : current methods. Wymondham: Horizon Bioscience. ISBN 978-1-904933-30-4.
^ abcPadeken J, Zeller P, Gasser SM (April 2015). "Repeat DNA in genome organization and stability". Current Opinion in Genetics & Development. 31: 12–19. doi:10.1016/j.gde.2015.03.009. PMID25917896.
^ abUsdin K (July 2008). "The biological effects of simple tandem repeats: lessons from the repeat expansion diseases". Genome Research. 18 (7): 1011–19. doi:10.1101/gr.070409.107. PMC3960014. PMID18593815.
^Li YC, Korol AB, Fahima T, Beiles A, Nevo E (December 2002). "Microsatellites: genomic distribution, putative functions and mutational mechanisms: a review". Molecular Ecology. 11 (12): 2453–65. doi:10.1046/j.1365-294X.2002.01643.x. PMID12453231.
^ abWessler SR (November 2006). "Transposable elements and the evolution of eukaryotic genomes". Proceedings of the National Academy of Sciences of the United States of America. 103 (47): 17600–01. Bibcode:2006PNAS..10317600W. doi:10.1073/pnas.0607612103. PMC1693792. PMID17101965.
^Deininger PL, Moran JV, Batzer MA, Kazazian HH (December 2003). "Mobile elements and mammalian genome evolution". Current Opinion in Genetics & Development. 13 (6): 651–58. doi:10.1016/j.gde.2003.10.013. PMID14638329.
^Kidwell MG, Lisch DR (March 2000). "Transposable elements and host genome evolution". Trends in Ecology & Evolution. 15 (3): 95–99. doi:10.1016/S0169-5347(99)01817-0. PMID10675923.
^Richard GF, Kerrest A, Dujon B (December 2008). "Comparative genomics and molecular dynamics of DNA repeats in eukaryotes". Microbiology and Molecular Biology Reviews. 72 (4): 686–727. doi:10.1128/MMBR.00011-08. PMC2593564. PMID19052325.
^Cordaux R, Batzer MA (October 2009). "The impact of retrotransposons on human genome evolution". Nature Reviews. Genetics. 10 (10): 691–703. doi:10.1038/nrg2640. PMC2884099. PMID19763152.
^Han JS, Boeke JD (August 2005). "LINE-1 retrotransposons: modulators of quantity and quality of mammalian gene expression?". BioEssays. 27 (8): 775–84. doi:10.1002/bies.20257. PMID16015595.
^Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA, Smith HO, Venter JC (January 2006). "Essential genes of a minimal bacterium". Proceedings of the National Academy of Sciences of the United States of America. 103 (2): 425–30. Bibcode:2006PNAS..103..425G. doi:10.1073/pnas.0510013103. PMC1324956. PMID16407165.
^Forster AC, Church GM (2006). "Towards synthesis of a minimal cell". Molecular Systems Biology. 2 (1): 45. doi:10.1038/msb4100090. PMC1681520. PMID16924266.
^Mankertz P (2008). "Molecular Biology of Porcine Circoviruses". Animal Viruses: Molecular Biology. Caister Academic Press. ISBN 978-1-904455-22-6.
^Fiers W, Contreras R, Duerinck F, Haegeman G, Iserentant D, Merregaert J, Min Jou W, Molemans F, Raeymaekers A, Van den Berghe A, Volckaert G, Ysebaert M (April 1976). "Complete nucleotide sequence of bacteriophage MS2 RNA: primary and secondary structure of the replicase gene". Nature. 260 (5551): 500–07. Bibcode:1976Natur.260..500F. doi:10.1038/260500a0. PMID1264203.
^Fiers W, Contreras R, Haegemann G, Rogiers R, Van de Voorde A, Van Heuverswyn H, Van Herreweghe J, Volckaert G, Ysebaert M (May 1978). "Complete nucleotide sequence of SV40 DNA". Nature. 273 (5658): 113–20. Bibcode:1978Natur.273..113F. doi:10.1038/273113a0. PMID205802.
^Sanger F, Air GM, Barrell BG, Brown NL, Coulson AR, Fiddes CA, Hutchison CA, Slocombe PM, Smith M (February 1977). "Nucleotide sequence of bacteriophage phi X174 DNA". Nature. 265 (5596): 687–95. Bibcode:1977Natur.265..687S. doi:10.1038/265687a0. PMID870828.
^"Virology – Human Immunodeficiency Virus And Aids, Structure: The Genome And Proteins Of HIV". Pathmicro.med.sc.edu. 1 July 2010. Retrieved 27 January 2011.
^Thomason L, Court DL, Bubunenko M, Costantino N, Wilson H, Datta S, Oppenheim A (April 2007). "Recombineering: genetic engineering in bacteria using homologous recombination". Current Protocols in Molecular Biology. Chapter 1: Unit 1.16. doi:10.1002/0471142727.mb0116s78. ISBN 978-0-471-14272-0. PMID18265390. S2CID 490362.
^Court DL, Oppenheim AB, Adhya SL (January 2007). "A new look at bacteriophage lambda genetic networks". Journal of Bacteriology. 189 (2): 298–304. doi:10.1128/JB.01215-06. PMC1797383. PMID17085553.
^Sanger F, Coulson AR, Hong GF, Hill DF, Petersen GB (December 1982). "Nucleotide sequence of bacteriophage lambda DNA". Journal of Molecular Biology. 162 (4): 729–73. doi:10.1016/0022-2836(82)90546-0. PMID6221115.
^Legendre M, Arslan D, Abergel C, Claverie JM (January 2012). "Genomics of Megavirus and the elusive fourth domain of Life". Communicative & Integrative Biology. 5 (1): 102–06. doi:10.4161/cib.18624. PMC3291303. PMID22482024.
^Philippe N, Legendre M, Doutre G, Couté Y, Poirot O, Lescot M, Arslan D, Seltzer V, Bertaux L, Bruley C, Garin J, Claverie JM, Abergel C (July 2013). "Pandoraviruses: amoeba viruses with genomes up to 2.5 Mb reaching that of parasitic eukaryotes" (PDF). Science. 341 (6143): 281–86. Bibcode:2013Sci...341..281P. doi:10.1126/science.1239181. PMID23869018. S2CID 16877147.
^Bennett GM, Moran NA (5 August 2013). "Small, smaller, smallest: the origins and evolution of ancient dual symbioses in a Phloem-feeding insect". Genome Biology and Evolution. 5 (9): 1675–88. doi:10.1093/gbe/evt118. PMC3787670. PMID23918810.
^Shigenobu S, Watanabe H, Hattori M, Sakaki Y, Ishikawa H (September 2000). "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS". Nature. 407 (6800): 81–86. Bibcode:2000Natur.407...81S. doi:10.1038/35024074. PMID10993077.
^Fleischmann RD, Adams MD, White O, Clayton RA, Kirkness EF, Kerlavage AR, Bult CJ, Tomb JF, Dougherty BA, Merrick JM (July 1995). "Whole-genome random sequencing and assembly of Haemophilus influenzae Rd". Science. 269 (5223): 496–512. Bibcode:1995Sci...269..496F. doi:10.1126/science.7542800. PMID7542800. S2CID 10423613.
^Blattner FR, Plunkett G, Bloch CA, Perna NT, Burland V, Riley M, et al. (September 1997). "The complete genome sequence of Escherichia coli K-12". Science. 277 (5331): 1453–62. doi:10.1126/science.277.5331.1453. PMID9278503.
^Challacombe JF, Eichorst SA, Hauser L, Land M, Xie G, Kuske CR (15 September 2011). Steinke D (ed.). "Biological consequences of ancient gene acquisition and duplication in the large genome of Candidatus Solibacter usitatus Ellin6076". PLOS ONE. 6 (9): e24882. Bibcode:2011PLoSO...624882C. doi:10.1371/journal.pone.0024882. PMC3174227. PMID21949776.
Dufresne A, Salanoubat M, Partensky F, Artiguenave F, Axmann IM, Barbe V, et al. (August 2003). "Genome sequence of the cyanobacterium Prochlorococcus marinus SS120, a nearly minimal oxyphototrophic genome". Proceedings of the National Academy of Sciences of the United States of America. 100 (17): 10020–25. Bibcode:2003PNAS..10010020D. doi:10.1073/pnas.1733211100. PMC187748. PMID12917486.
Meeks JC, Elhai J, Thiel T, Potts M, Larimer F, Lamerdin J, Predki P, Atlas R (2001). "An overview of the genome of Nostoc punctiforme, a multicellular, symbiotic cyanobacterium". Photosynthesis Research. 70 (1): 85–106. doi:10.1023/A:1013840025518. PMID16228364.
^Parfrey LW, Lahr DJ, Katz LA (April 2008). "The dynamic nature of eukaryotic genomes". Molecular Biology and Evolution. 25 (4): 787–94. doi:10.1093/molbev/msn032. PMC2933061. PMID18258610.
^ScienceShot: Biggest Genome Ever Archived 11 October 2010 at the Wayback Machine, comments: "The measurement for Amoeba dubia and other protozoa which have been reported to have very large genomes were made in the 1960s using a rough biochemical approach which is now considered to be an unreliable method for accurate genome size determinations."
^Anderson S, Bankier AT, Barrell BG, de Bruijn MH, Coulson AR, Drouin J, Eperon IC, Nierlich DP, Roe BA, Sanger F, Schreier PH, Smith AJ, Staden R, Young IG (April 1981). "Sequence and organization of the human mitochondrial genome". Nature. 290 (5806): 457–65. Bibcode:1981Natur.290..457A. doi:10.1038/290457a0. PMID7219534.
^Fleischmann A, Michael TP, Rivadavia F, Sousa A, Wang W, Temsch EM, Greilhuber J, Müller KF, Heubl G (December 2014). "Evolution of genome size and chromosome number in the carnivorous plant genus Genlisea (Lentibulariaceae), with a new estimate of the minimum genome size in angiosperms". Annals of Botany. 114 (8): 1651–63. doi:10.1093/aob/mcu189. PMC4649684. PMID25274549.
^"Genome Assembly". The Arabidopsis Information Resource (TAIR).
^Greilhuber J, Borsch T, Müller K, Worberg A, Porembski S, Barthlott W (November 2006). "Smallest angiosperm genomes found in lentibulariaceae, with chromosomes of bacterial size". Plant Biology. 8 (6): 770–77. doi:10.1055/s-2006-924101. PMID17203433.
^Tuskan GA, Difazio S, Jansson S, Bohlmann J, Grigoriev I, Hellsten U, et al. (September 2006). "The genome of black cottonwood, Populus trichocarpa (Torr. & Gray)" (PDF). Science. 313 (5793): 1596–604. Bibcode:2006Sci...313.1596T. doi:10.1126/science.1128691. PMID16973872.
^Pellicer J, Fay MF, Leitch IJ (15 September 2010). "The largest eukaryotic genome of them all?". Botanical Journal of the Linnean Society. 164 (1): 10–15. doi:10.1111/j.1095-8339.2010.01072.x.
^Lang D, Zimmer AD, Rensing SA, Reski R (October 2008). "Exploring plant biodiversity: the Physcomitrella genome and beyond". Trends in Plant Science. 13 (10): 542–49. doi:10.1016/j.tplants.2008.07.002. PMID18762443.
^"Saccharomyces Genome Database". Yeastgenome.org. Retrieved 27 January 2011.
^Galagan JE, Calvo SE, Cuomo C, Ma LJ, Wortman JR, Batzoglou S, et al. (December 2005). "Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae". Nature. 438 (7071): 1105–15. Bibcode:2005Natur.438.1105G. doi:10.1038/nature04341. PMID16372000.
^Leroy S, Bouamer S, Morand S, Fargette M (2007). "Genome size of plant-parasitic nematodes". Nematology. 9 (3): 449–50. doi:10.1163/156854107781352089.
^The C. elegans Sequencing Consortium (December 1998). "Genome sequence of the nematode C. elegans: a platform for investigating biology". Science. 282 (5396): 2012–18. Bibcode:1998Sci...282.2012.. doi:10.1126/science.282.5396.2012. PMID9851916. S2CID 16873716.
^Ellis LL, Huang W, Quinn AM, Ahuja A, Alfrejd B, Gomez FE, Hjelmen CE, Moore KL, Mackay TF, Johnston JS, Tarone AM (July 2014). "Intrapopulation genome size variation in D. melanogaster reflects life history variation and plasticity". PLOS Genetics. 10 (7): e1004522. doi:10.1371/journal.pgen.1004522. PMC4109859. PMID25057905.
^Honeybee Genome Sequencing Consortium (October 2006). "Insights into social insects from the genome of the honeybee Apis mellifera". Nature. 443 (7114): 931–49. Bibcode:2006Natur.443..931T. doi:10.1038/nature05260. PMC2048586. PMID17073008.
^The International Silkworm Genome (December 2008). "The genome of a lepidopteran model insect, the silkworm Bombyx mori". Insect Biochemistry and Molecular Biology. 38 (12): 1036–45. doi:10.1016/j.ibmb.2008.11.004. PMID19121390.
^Wurm Y, Wang J, Riba-Grognuz O, Corona M, Nygaard S, Hunt BG, et al. (April 2011). "The genome of the fire ant Solenopsis invicta". Proceedings of the National Academy of Sciences of the United States of America. 108 (14): 5679–84. Bibcode:2011PNAS..108.5679W. doi:10.1073/pnas.1009690108. PMC3078418. PMID21282665.
^Church DM, Goodstadt L, Hillier LW, Zody MC, Goldstein S, She X, et al. (May 2009). Roberts RJ (ed.). "Lineage-specific biology revealed by a finished genome assembly of the mouse". PLOS Biology. 7 (5): e1000112. doi:10.1371/journal.pbio.1000112. PMC2680341. PMID19468303.
^"Human Genome Project Information Site Has Been Updated". Ornl.gov. 23 July 2013. Archived from the original on 20 September 2008. Retrieved 6 February 2014.
^Venter JC, Adams MD, Myers EW, Li PW, Mural RJ, Sutton GG, et al. (February 2001). "The sequence of the human genome". Science. 291 (5507): 1304–51. Bibcode:2001Sci...291.1304V. doi:10.1126/science.1058040. PMID11181995.
^"Pan paniscus (pygmy chimpanzee)". nih.gov. Retrieved 30 June 2016.
^International Chicken Genome Sequencing Consortium (December 2004). "Sequence and comparative analysis of the chicken genome provide unique perspectives on vertebrate evolution". Nature. 432 (7018): 695–716. Bibcode:2004Natur.432..695C. doi:10.1038/nature03154. ISSN 0028-0836. PMID15592404.
^Roest Crollius H, Jaillon O, Dasilva C, Ozouf-Costaz C, Fizames C, Fischer C, Bouneau L, Billault A, Quetier F, Saurin W, Bernot A, Weissenbach J (July 2000). "Characterization and repeat analysis of the compact genome of the freshwater pufferfish Tetraodon nigroviridis". Genome Research. 10 (7): 939–49. doi:10.1101/gr.10.7.939. PMC310905. PMID10899143.
^Jaillon O, Aury JM, Brunet F, Petit JL, Stange-Thomann N, Mauceli E, et al. (October 2004). "Genome duplication in the teleost fish Tetraodon nigroviridis reveals the early vertebrate proto-karyotype". Nature. 431 (7011): 946–57. Bibcode:2004Natur.431..946J. doi:10.1038/nature03025. PMID15496914.
^"Tetraodon Project Information". Archived from the original on 26 September 2012. Retrieved 17 October 2012.
^Martincorena I, Campbell PJ (September 2015). "Somatic mutation in cancer and normal cells". Science. 349 (6255): 1483–89. Bibcode:2015Sci...349.1483M. doi:10.1126/science.aab4082. PMID26404825.
^Hajkova P, Jeffries SJ, Lee C, Miller N, Jackson SP, Surani MA (July 2010). "Genome-wide reprogramming in the mouse germ line entails the base excision repair pathway". Science. 329 (5987): 78–82. Bibcode:2010Sci...329...78H. doi:10.1126/science.1187945. PMC3863715. PMID20595612.
^Hackett JA, Sengupta R, Zylicz JJ, Murakami K, Lee C, Down TA, Surani MA (January 2013). "Germline DNA demethylation dynamics and imprint erasure through 5-hydroxymethylcytosine". Science. 339 (6118): 448–52. Bibcode:2013Sci...339..448H. doi:10.1126/science.1229277. PMC3847602. PMID23223451.