Jackie O often refers to Jacqueline Onassis, who was the wife of the 35th President of the United States, John F. Kennedy.

It may also refer to:

• Jackie O (opera), a 1997 opera by Michael Daugherty and Wayne Koestenbaum
• Jackie O (radio host) (born 1975), Jacqueline Ellen Marie Henderson, an Australian television presenter and radio personality
• Jacki-O (born 1975), American rapper
• Jackie-O Motherfucker, an American experimental rock group