The short features Jasper, his friend/nemesis Professor Scarecrow, and Blackbird.^[4] The short opens with a brief view of the hovel where Jasper lives with his mother, then the camera moves to the chicken coop. Jasper's mother counts her chickens and realizes there is another one missing. She has realized that she is facing a chicken thief. Nearby, said thief, the Scarecrow, is seen finishing his meal.^[4]

Next Jasper's mother prepares to leave her residence. She first arms Jasper and tasks him with guarding the chickens. Jasper fancies himself a soldier, and starts marching within his residence. When the Scarecrow appears at his window, Jasper immediately points the weapon at him. The Scarecrow is at first terrified. Then explains to Jasper that he should not be pointing the weapon at live targets, since it is likely to go off. Convincing the boy to hand him the gun, the Scarecrow claims that the "mean-looking" weapon reminds him of his past as a big game hunter.^[4]

As the Scarecrow begins narrating his supposed past at the Belgian Congo, the scenery changes around the characters. The interior of the hovel is replaced by a jungle environment. The trio are off to a hunting expedition. As the Scarecrow explains that they ate after big game, he fails to notice an African elephant standing behind him. When he does notice, the "brave" hunter flees in terror.^[4] In a 23-second scene, the Scarecrow points his gun at a rabbit hole and orders its resident to come out. Bugs Bunny emerges, notices his surrounding and realizes that he is in "the wrong picture", and returns to his hole.^[4]

A confrontation with an enraged elephant sends the trio flying back towards the hovel. The Scarecrow lands in the chicken coop and is soon confronted by Jasper's mother, who is also armed and figures that this guy is the chicken thief. The final scenes has the Scarecrow and Blackbird in prison.^[4]

The Jasper series of shorts relied on a small, consistent cast. The titular character was a playful pickaninny, his mother a protective mammy, Professor Scarecrow being a black scam artist, and the Blackbird serving as his fast-talking partner-in-crime.^[5] Pal described his protagonist as a Huckleberry Finn influenced by African-American folktales. Considering that the righteous boy consistently managed to outwit his plotting enemies, one can see some resemblance to Br'er Rabbit.^[6]

The series' use of stereotypes did not seem to attract much criticism at first, but in 1946, Ebony reported "Negro" groups protesting their depiction by Pal. In an apparent response to the criticism, Pal released John Henry and the Inky-Poo (1946), a short based on the folk hero John Henry. Ebony responded favorably at this more serious depiction of a "Negro" protagonist.^[6]

• Catalog of Copyright Entries. Part 1. [C] Group 3. Dramatic Composition and Motion Pictures. New Series, Library of Congress, United States Copyright Office, 1943–1944
• Cripps, Thomas (1977), Slow Fade to Black: The Negro in American Film, 1900–1942, Oxford University Press, ISBN 978-0-19-987845-1
• Cripps, Thomas (1993), Making Movies Black: The Hollywood Message Movie from World War II to the Civil Rights Era, Oxford University Press, ISBN 978-0-19-536034-9
• Lawson, Tim; Persons, Alisa (2004), "Mel Blanc", The Magic Behind the Voices: A Who's Who of Cartoon Voice Actors, University Press of Mississippi, ISBN 978-1-57806-696-4
• Sampson, Henry T. (1998), That's enough, folks: Black images in animated cartoons, 1900–1960, Scarecrow Press, ISBN 978-0-8108-3250-3