Peggy Martin and her mother Mae both work as burlesque chorus girls. After star Bubbles LaRue quits, stage manager Joe asks Mae to perform a special number, but Mae secretly arranges for Peggy to take the opportunity. Peggy's performance is so good that she is offered the starring spot.

One evening, Randy Carroll, a member of a wealthy society family in Cleveland, is brought to a performance by friends and becomes enamored of Peggy. Learning that Peggy generally does not accept dates because her mother disapproves, Randy adopts a subtle strategy. Every night, he sends Peggy an orchid, but does not sign the card. Curious about her secret admirer, Peggy asks the florist for the sender's identity. When the florist tells her that the man is due to arrive at any moment, Peggy waits for him. After they finally meet, Randy asks Peggy to dinner and she accepts, but she first invites him to meet her mother. Randy is surprised to learn that Mae is also a dancer, but he asks her to join them for dinner. Mae declines, but waits anxiously for Peggy to return home. When Peggy appears, she excitedly informs Mae that Randy has proposed marriage.

The next day, when Randy asks Mae for her consent, she warns him that there is a class difference between him and Peggy. In response to Randy's indifference, Mae recounts the story of her marriage to a Boston socialite, Peggy's father. After their marriage, her husband's family was horrified to learn how she made her living and had the marriage annulled. Randy protests that people are more broadminded now than they were in her day, and Mae agrees to the marriage on the condition that Randy tells his mother about Peggy's profession beforehand.

Randy fails to summon the courage to tell his mother Adele about Peggy's profession. Adele is delighted that Randy has fallen in love and invites Mae and Peggy for a visit. Adele plans a lavish engagement party for all of their friends, including Mae's old friend Billy Mackay, a retired burlesque comic who has loved Mae for years. At the party, the trio of musicians that Adele has hired to entertain recognize Peggy and ask her to sing, addressing her as "Peggy, the Queen of Burlesque" in front of all of the guests. The guests are scandalized, and feeling snubbed, Peggy and Mae attempt to leave, but Adele stops them, declaring that if they run away it will only make things worse.

Adele asks Billy to help her sing a song with the trio. Later, she announces to her shocked friends that she too had been a chorus girl. Billy secretly tells Mae that Adele had concocted the story to help Randy and Peggy. As Randy and Peggy embrace, Adele persuades Billy and Mae to make it a double wedding.

• Adele Jergens as Mae Martin
• Marilyn Monroe as Peggy Martin
• Rand Brooks as Randy Carroll
• Nana Bryant as Mrs. Adele Carroll
• Eddie Garr as Billy Mackay
• Steven Geray as Salisbury
• The Bobby True Trio as Trio Musicians
• Kathleen O'Malley as Nita
• Dave Barry as Ripple the Decorator (uncredited)

Five of songs featured in the film were written by Lester Lee and Allan Roberts.^[1] The song "Ubangi Love Song" performed by the Bobby True Trio was written by Buck Ram.

• Ladies of the Chorus at IMDb  
• Ladies of the Chorus at AllMovie
• Ladies of the Chorus at the TCM Movie Database
• Ladies of the Chorus at the American Film Institute Catalog
• Ladies of the Chorus at Rotten Tomatoes