Works in the Palatine Gallery at the Palazzo Pitti in Florence, Italy include:

• Raphael, Madonna of the Grand Duke
• Raphael, The Madonna of the Chair
• Raphael, Portrait of Pope Leo X and two Cardinals
• Raphael, companion portraits of Agnolo Doni and his wife, Maddelena Doni, for whom Michelangelo's Doni Tondo was commissioned.
• Andrea del Sarto, The Young John the Baptist
• Andrea del Sarto, Disputation on the Holy Trinity
• Sebastiano del Piombo, Martyrdom of St Agatha
• Titian, Mary Magdalene
• Titian, Portrait of Pietro Aretino
• Titian, Portrait of an Englishman, perhaps a member of the Howard family, (also known as The Man with Blue Eyes).
• Titian, Penitent Magdalene
• Murillo, Madonna and Child
• Rubens, a group portrait known as The four Philosophers
• Rubens, Allegory of War
• Caravaggio, The sleeping Cupid
• Artemisia Gentileschi, Judith
• Parmigianino, Madonna with the long neck
• Anthony van Dyck, Portrait of Cardinal Guido Bentivoglio
• Velázquez, Philip IV of Spain on Horseback