Nicholas II was the last emperor of Russia from 1894 to 1917.

Nicholas II may also refer to:

• Pope Nicholas II (c. 990/995–1061)
• Nicholas II of Transylvania (1213), voivode
• Nicholas II of Saint Omer (r. 1258–1294)
• Patriarch Nicholas II of Alexandria (r. 1263–1276)
• Nicholas II, Duke of Opava (1268–1365)
• Nicholas II Garay (1367–1433)
• Nicholas II, Count of Tecklenburg (died 1426)
• Nicholas II Zorzi (14th–15th century)
• Nicholas II of Niemodlin (c. 1462–1497)
• Nicolaus II Bernoulli, Swiss mathematician (1695–1726)
• Nicholas II, Prince Esterházy (1765–1833)
• Crown Prince Nicholas II of Montenegro (born 1944)