The Prairies and Pineywoods Wildlife Trail is a state-designated system of trails and wildlife sanctuaries in the Texas Panhandle in the United States. It is one of the four major wildlife trail systems designated by the State of Texas.

The trail system consists of two separate groups of rails. The western trails, which are more prairie terrain, cover an area stretching from Brenham and College Station in the south to Wichita Falls and Denison in the north. The eastern trails, which are more woodland terrain, cover an area stretching from Huntsville and Hemphill in the south to Paris and Texarkana in the north.

The trails include locations in Sam Houston National Forest, Davy Crockett National Forest, and Sabine National Forest.