He is best known for his two near life-size copies from the early 1590s of The Family of Sir Thomas More (1527) by Hans Holbein the Younger. The original is now lost–it was destroyed by fire in the eighteenth century.^[3] Lockey's versions, which are at Nostell Priory and the National Portrait Gallery in London, differ considerably, as the London version, Sir Thomas More, his father, his household and his descendants, includes More's descendants in their contemporary dress but omits several of the figures in the Holbein original. This was painted around 1593, probably commissioned by More's grandson, Thomas More II, to commemorate five generations of the family. The four figures wearing ruffs and holding prayer books on the right are Thomas More II, his wife and their oldest and youngest sons. Anne More, née Cresacre (1511–77), was Sir Thomas's daughter-in-law, and appears twice: once copied from the Holbein as a young woman of about sixteen (between Sir Thomas and his father) and also as an older woman in the painting on the rear wall.^[5] A cabinet miniature version of this portrait c. 1594 with different details, also likely by Lockey, is in the Victoria and Albert Museum.^[3][6] There is also a surviving drawing by Holbein which confirms the general accuracy of the Nostell Priory version.

Two further copies of the Holbein, at old Chelsea Town Hall and Hendred House, East Hendred, may be by Lockey, but are too damaged and over-painted for any certainty to be possible.^[7]

His signed portrait of Lady Margaret Beaufort, mother of Henry VII, based on a work by Meynnart Wewyck (Maynard Vewicke) was presented to St John's College, Cambridge in 1598,^[3] and a portrait of King James I of England as a young boy, now in the National Portrait Gallery, London, based on a 1574 painting by Arnold Bronckorst, is also attributed to Lockey^[8][9]

Lockey was long associated with the Cavendish family of Hardwick Hall, working under the patronage of Bess of Hardwick between 1591 and 1597 and of her son Sir William Cavendish between 1608 and 1613.^[1][10] He produced around forty life-size pictures including copies of portraits of Cavendish's Elizabethan ancestors.^[11]

The art historian Sir Roy Strong has identified a number of inferior copies of Hilliard's portrait miniatures as probably the work of Lockey, pointing out their "weak and laboured brushstrokes" and "smudgy" features.^[12] Strong concludes that while Lockey was of "no significance as an artist"^[13] when compared to his brilliant fellow-apprentice to Hilliard Isaac Oliver, his importance lies in carrying Hilliard's aesthetic into the next generation.

Lockey can be associated with Dutch or Flemish painters residing in London in the 1580s. One such painter, Peter Matheeusen in his 1588 will made bequests to his cousin Adrian Vanson, to the miniaturist Isaac Oliver and to Rowland Lockey, including his library of manuals for painters.^[14]

• Artists of the Tudor court
• List of British artists

• Hearn, Karen, ed. (1995).Dynasties: Painting in Tudor and Jacobean England 1530-1630. New York, Rizzoli. ISBN 0-8478-1940-X.
• Lesley Lewis (1998). Rowland Lockey (1565/7–1616). Gracewing Publishing. ISBN 9780852444665. Retrieved 2 November 2008. {{cite book}}: |work= ignored (help) ISBN 0-85244-466-4
• Strong, Roy (1969). The English Icon: Elizabethan and Jacobean Portraiture, London, Routledge & Kegan Paul
• Strong, Roy (983). The English Renaissance Miniature, New York, Thames and Hudson, ISBN 0-500-23370-5

• Keys to the figures in the various More family versions
• 13 artworks by or after Rowland Lockey at the Art UK site