After excision the choroid collapses partially, histologic preparations also alter the local pressure and fluid content of different sections in the tissue, thus requiring preparations with rubber solution or others that can conserve the vascular status of living tissue. Novel diagnostic methods, especially optical coherence tomography have widened the understanding of the real-time, in vivo status of the different layers.
Several papers have shown the relationship between the thickness of the choroidal, Sattler's and Haller's layer between healthy individuals and in people with age-related macular degeneration (AMD). The studies showed significant reduction of layer thickness in relation to the progression of AMD, which may be important in the understanding of choriopathy in the pathophysiology of AMD. However, also strong variations even throughout the diurnal cycle, as well as the influence of optical stimuli during eye-growth, indicate that the complex function of this tissue is not entirely understood and might be one of the reasons for the frequently found separation in vascular size between Haller's and Sattler's layer.
^Some authors consider the vascular region of the choroid as being two separate layers, namely Sattler's and Haller's layer, and some consider the lamina fusca as being either of scleral or choroidal origin.
^Sattler, Hubert (1876). "Ueber den Feineren Bau der Chorioidea des Menschen nebst Beitraegen zur Pathologischen und Vergleichenden Anatomie der Aderhaut". Albrecht von Graefe's Archiv für Ophthalmologie. 22 (2): 1–100. doi:10.1007/bf01705015. Retrieved 7 January 2017.
^L. Nickla, Debora; Wallman, Josh (2010). "The Multifunctional Choroid". Progress in Retinal and Eye Research. 29 (2): 144–168. doi:10.1016/j.preteyeres.2009.12.002. PMC2913695. PMID20044062.
^Heimann, K. (1976-01-01). "The Development of the Choroidal Vascular System". In Laey, J. J. De (ed.). International Symposium on Fluorescein Angiography Ghent 28 March—1 April 1976. Documenta Ophthalmologica Proceedings Series. Vol. 9. Springer Netherlands. pp. 181–186. doi:10.1007/978-94-010-1573-8_31. ISBN 9789061931492.
^Howard, Harvey (1917-01-01). "A Case Showing Multiple Congenital Abnormalities of the Eye; the Origin of the Vitreous Indicated by One of Them". Transactions of the American Ophthalmological Society. 15: 244–301. ISSN 0065-9533. PMC1318126. PMID16692420.
^Považay, Boris; Bizheva, K.; Hermann, B.; Unterhuber, A.; Sattmann, H.; Fercher, A.; Drexler, W.; Schubert, C.; Ahnelt, P. (2003-08-25). "Enhanced visualization of choroidal vessels using ultrahigh resolution ophthalmic OCT at 1050 nm" (PDF). Optics Express. 11 (17): 1980–1986. Bibcode:2003OExpr..11.1980P. doi:10.1364/oe.11.001980. ISSN 1094-4087. PMID19466083.
^Esmaeelpour, M.; Ansari-Shahrezaei, S.; Glittenberg, C.; Nemetz, S.; Kraus, M.F. (22 July 2014). "Choroid, Haller's, and Sattler's layer thickness in intermediate age-related macular degeneration with and without fellow neovascular eyes". Investigative Ophthalmology & Visual Science. 55 (8): 5074–80. doi:10.1167/iovs.14-14646. PMC4132555. PMID25052997.
^Brown, Jamin S.; Flitcroft, D. Ian; Ying, Gui-shuang; Francis, Ellie L.; Schmid, Gregor F.; Quinn, Graham E.; Stone, Richard A. (2009-01-01). "In Vivo Human Choroidal Thickness Measurements: Evidence for Diurnal Fluctuations". Investigative Ophthalmology & Visual Science. 50 (1): 5–12. doi:10.1167/iovs.08-1779. ISSN 1552-5783. PMC4112498. PMID18719079.
^Lam, Carly Siu Yin; Tang, Wing Chun; Tse, Dennis Yan-Yin; Tang, Ying Yung; To, Chi Ho (2014-01-01). "Defocus Incorporated Soft Contact (DISC) lens slows myopia progression in Hong Kong Chinese schoolchildren: a 2-year randomised clinical trial". British Journal of Ophthalmology. 98 (1): 40–45. doi:10.1136/bjophthalmol-2013-303914. ISSN 1468-2079. PMC3888618. PMID24169657.