VBI may refer to:

• Biocomplexity Institute of Virginia Tech (formerly the Virginia Bioinformatics Institute), a research organization specializing in bioinformatics, computational biology, and systems biology in Virginia, United States
• Value-based investing, also known as value investing, an investment paradigm that involves buying securities that appear underpriced by some form of fundamental analysis
• VBI Vaccines Inc. and Variation Biotechnologies, related manufacturers of vaccines
• Vertebrobasilar insufficiency, a temporary set of symptoms due to decreased blood flow (ischemia) in the posterior circulation of the brain
• Vertical blank interrupt, a hardware feature found in some computer systems that generate a video display
• vertical blanking interval, a portion of a television signal
• Visible Broadband Imager, part of the Daniel K. Inouye Solar Telescope