Water blue

Summary

Water blue, also known as aniline blue, Acid blue 22, Soluble Blue 3M, Marine Blue V, or C.I. 42755, is a chemical compound used as a stain in histology. Water blue stains collagen blue in tissue sections. It is soluble in water and slightly soluble in ethanol.

Water blue
Identifiers
  • 28631-66-5 checkY
3D model (JSmol)
  • Interactive image
ChemSpider
  • 21173243
ECHA InfoCard 100.044.634 Edit this at Wikidata
EC Number
  • 249-113-9
  • 139030972
UNII
  • 44D829LK4D checkY
  • DTXSID9044904 Edit this at Wikidata
  • InChI=1S/C32H27N3O9S3.2Na/c33-20-26-25(6-5-11-29(26)45(36,37)38)32(21-12-16-23(17-13-21)34-27-7-1-3-9-30(27)46(39,40)41)22-14-18-24(19-15-22)35-28-8-2-4-10-31(28)47(42,43)44;;/h1-19,34H,20,33H2,(H,36,37,38)(H,39,40,41)(H,42,43,44);;/q;2*+1/p-2
    Key: WJHQBPPQVMBLSW-UHFFFAOYSA-L
  • [H+].c1ccc(c(c1)Nc2ccc(cc2)C(=C3C=CC(=Nc4ccccc4S(=O)(=O)[O-])C=C3)c5cccc(c5CN)S(=O)(=O)[O-])S(=O)(=O)[O-].[Na+].[Na+]
Properties
C32H25N3O9S3Na2
Molar mass 737.72374 g/mol
Hazards
GHS labelling:
GHS07: Exclamation mark
Warning
H315, H317, H319, H335
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Infobox references

Water blue is also available in mixture with methyl blue, under the names Aniline Blue WS, Aniline blue, China blue, or Soluble blue.[1][2]

It can be used in the Mallory's trichrome stain of connective tissue and Gömöri trichrome stain of muscle tissue. It is used in differential staining.[3]

See also edit

References edit

  1. ^ Lillie, R. D. (1969). "H. J. Conn's Biological stains : a handbook on the nature and uses of the dyes employed in the biological laboratory". Trove (National Library of Australia). Retrieved February 8, 2019.
  2. ^ "Aniline Blue WS". StainsFile.
  3. ^ Nowicki, Marcin; et al. (15 May 2013), "A simple dual stain for detailed investigations of plant-fungal pathogen interactions", Vegetable Crops Research Bulletin, 77, Vegetable Crops Research bulleting, InHort & Versita: 61–74, doi:10.2478/v10032-012-0016-z