William Guthrie may refer to:

• William Guthrie (boxer) (born 1967), American boxer
• William Guthrie (historian) (1708–1770), Scottish writer and journalist
• William Guthrie (politician) (1884–1954), Canadian politician
• William Guthrie (minister) (1620–1665), Scottish Presbyterian minister and author
• William Dameron Guthrie (1859–1935), American lawyer
• W. K. C. Guthrie (William Keith Chambers Guthrie, 1906–1981), Scottish classical scholar and philosophy professor
• William Norman Guthrie (1868–1944), American clergyman
• William Tyrone Guthrie (1900–1971), English theatre director