Xiaoliang Sunney Xie


Xiaoliang Sunney Xie (Chinese: 谢晓亮; born 1962 in Beijing, China) is a Chinese American biochemist well known for his experiments in the fields of single-molecule biophysical chemistry and single-molecule enzymology.[2]

Xiaoliang Sunney Xie
Xiaoliang Sunney Xie 1.jpg
Born (1962-06-24) June 24, 1962 (age 60)
EducationBSc in Chemistry from Peking University
PhD in Physical Chemistry from UC San Diego
Known forSingle Molecule Enzymology,
Coherent Raman Imaging,
Single Cell Genomics
AwardsAlbany Prize in Medicine and Biomedical Research 2015,
Peter Debye Award in Physical Chemistry 2015,
Ellis R. Lippincott Award 2013,
Biophysical Society Founders Award 2012
NAS 2011
NAM 2016
CAS 2017
Scientific career
FieldsBiophysical Chemistry,
Optical Imaging,
InstitutionsHarvard University
Peking University
Doctoral advisorJohn Douglas Simon[1]

Early lifeEdit

He received his B.Sc. in Chemistry from Peking University in 1984, and his Ph.D. in Physical Chemistry in 1990 from University of California at San Diego. After a brief postdoctoral appointment at University of Chicago, he joined Pacific Northwest National Laboratory, where he rose from Senior Research Scientist to Chief Scientist. In 1998, he became the first tenured professor recruited by Harvard University among Chinese Scholars who came to the United States since Chinese economic reform.


He had been the Mallinckrodt Professor of Chemistry and Chemical Biology at Harvard University until 2018, when he became the Lee Shau-kee Professor of Peking University. Currently, he is also the Director of Biomedical Pioneering Innovation Center (BIOPIC), and the Director of Beijing Advanced Innovation Center for Genomics (ICG), both at Peking University.

As a pioneer of single-molecule biophysical chemistry, Coherent Raman scattering microscopy, and single-cell genomics, he made major contributions to the emergence of these fields. Furthermore, he has made significant advances on medical applications of label-free optical imaging and single-cell genomics. In particular, his inventions in single-cell genomics have been used in in vitro fertilization to benefit hundreds of couples in China by avoiding the transmission of monogenic diseases to their newborns.

As of Jan 2019, Xie has published over 226 papers. More than forty of his students and post-doctorates have become professors at major universities around the world, and two are co-founders of start-up companies. Professor Xie’s current research interests include the following scientific, technological, and medical areas:

Honors and awardsEdit

Selected LiteratureEdit

Single-Cell GenomicsEdit

  • Tan, Longzhi; Xie, X. Sunney (2018). "A Near-complete Spatial Map of Olfactory Receptors in the Mouse Main Olfactory Epithelium". Chem Senses. 43 (6): 427–432. doi:10.1093/chemse/bjy030. PMC 6454507. PMID 29796642.
  • Xu, Juanjuan; Fang, Rui; Chen, Li; Chen, Daozhen; Xiao, Jian-Ping; Yang, Weimin; Wang, Honghua; Song, Xiaoqing; Ma, Ting; Bo, Shiping; Shi, Chong; Ren, Jun; Huang, Lei; Cai, Li-Yi; Yao, Bing; Xie, X Sunney; Lu, Sijia (2016). "Noninvasive chromosome screening of human embryos by genome sequencing of embryo culture medium for in vitro fertilization". Proc Natl Acad Sci USA. 113 (42): 11907–1. doi:10.1073/pnas.1613294113. PMC 5081593. PMID 27688762.
  • Chen, Chongyi; Xing, Dong; Tan, Longzhi; Li, Heng; Zhou, Guangyu; Huang, Lei; Xie, X. Sunney (2017). "Single-cell whole-genome analyses by Linear Amplification via Transposon Insertion (LIANTI)". Science. 356 (6334): 189–194. Bibcode:2017Sci...356..189C. doi:10.1126/science.aak9787. PMC 5538131. PMID 28408603.
  • Yan, Liying; Huang, Lei; Xu, Liya; Huang, Jin; Ma, Fei; Zhu, Xiaohui; Tang, Yaqiong; Liu, Mingshan; Lian, Ying; Liu, Ping; Li, Rong; Lu, Sijia; Tang, Fuchou; Qiao, Jie; Xie, X Sunney (2015). "Live births after simultaneous avoidance of monogenic diseases and chromosome abnormality by next-generation sequencing with linkage analyses". Proc Natl Acad Sci USA. 112 (52): 15964–15969. Bibcode:2015PNAS..11215964Y. doi:10.1073/pnas.1523297113. PMC 4702982. PMID 26712022.
  • Hou, Yu; Fan, Wei; Yan, Liying; Li, Rong; Lian, Ying; Huang, Jin; Li, Jinsen; Xu, Liya; Tang, Fuchou; Xie, X. Sunney; Qiao, Jie (2013). "Genome Analyses of Single Human Oocytes". Cell. 155 (7): 1492–1506. doi:10.1016/j.cell.2013.11.040. PMID 24360273.
  • Zong, Chenghang; Lu, Sijia; Chapman, Alec R.; Xie, X. Sunney (2012). "Genome-Wide Detection of Single-Nucleotide and Copy-Number Variations of a Single Human Cell". Science. 338 (6114): 1622–1626. Bibcode:2012Sci...338.1622Z. doi:10.1126/science.1229164. PMC 3600412. PMID 23258894.

Gene Expression and RegulationEdit

  • Chong, Shasha; Chen, Chongyi; Ge, Hao; Sunney, Xie X. (2014). "Mechanism of Transcriptional Bursting in Bacteria". Cell. 158 (2): 314–326. doi:10.1016/j.cell.2014.05.038. PMC 4105854. PMID 25036631.
  • Taniguchi, Yuichi; Choi, Paul J.; Li, Gene-Wei; Chen, Huiyi; Babu, Mohan; Hearn, Jeremy; Emili, Andrew; Xie, X. Sunney (2010). "Quantifying E. coli Proteome and Transcriptome with Single-Molecule Sensitivity in Single Cells". Science. 329 (5991): 533–538. Bibcode:2010Sci...329..533T. doi:10.1126/science.1188308. PMC 2922915. PMID 20671182.
  • Choi, Paul J.; Cai, Long; Frieda, Kirsten; Xie, X. Sunney (2008). "A Stochastic Single-Molecule Event Triggers Phenotype Switching of a Bacterial Cell". Science. 322 (5900): 442–446. Bibcode:2008Sci...322..442C. doi:10.1126/science.1161427. PMC 2819113. PMID 18927393.
  • Elf, Johan; Li, Gene-Wei; Xie, X. Sunney (2007). "Probing Transcription Factor Dynamics at the Single-Molecule Level in a Living Cell". Science. 316 (5828): 1191–1194. Bibcode:2007Sci...316.1191E. doi:10.1126/science.1141967. PMC 2853898. PMID 17525339.
  • Yu, Ji; Xiao, Jie; Ren, Xiaojia; Lao, Kaiqin; Xie, X. Sunney (2006). "Probing Gene Expression in Live Cells, One Protein Molecule at a Time". Science. 311 (5767): 1600–1603. Bibcode:2006Sci...311.1600Y. doi:10.1126/science.1119623. PMID 16543458. S2CID 14506807.
  • Cai, Long; Friedman, Nir; Xie, X. Sunney (2006). "Stochastic Protein Expression in Individual Cells at the Single Molecule Level". Nature. 440 (7082): 358–362. Bibcode:2006Natur.440..358C. doi:10.1038/nature04599. PMID 16541077. S2CID 4426109.

Single Molecule EnzymologyEdit

  • Chong, Shasha; Chen, Chongyi; Ge, Hao; Sunney, Xie X. (2014). "Mechanism of Transcriptional Bursting in Bacteria". Cell. 158 (2): 314–326. doi:10.1016/j.cell.2014.05.038. PMC 4105854. PMID 25036631.
  • Kim, Sangjin; Broströmer, Erik; Xing, Dong; Jin, Jianshi; Chong, Shasha; Ge, Hao; Wang, Siyuan; Gu, Chan; Yang, Lijiang; Gao, Yiqin; Su, Xiao-dong; Sun, Yujie; Xie, X. Sunney (2013). "Probing Allostery Through DNA". Science. 339 (6121): 816–819. Bibcode:2013Sci...339..816K. doi:10.1126/science.1229223. PMC 3586787. PMID 23413354.
  • English, Brian P.; Min, Wei; van Oijen, Antoine M.; Lee, Kang Taek; Luo, Guobin; Sun, Hongye; Cherayil, Binny J.; Kou, S.C.; Xie, X. Sunney (2006). "Ever-Fluctuating Single Enzyme Molecules: Michaelis-Menten Equation Revisited". Nat Chem Biol. 2 (2): 87–94. doi:10.1038/nchembio759. PMID 16415859. S2CID 2201882.
  • Yang, Haw; Luo, Guobin; Karnchanaphanurach, Pallop; Louie, Tai-Man; Rech, Ivan; Cova, Sergio; Xun, Luying; Xie, X. Sunney (2003). "Protein Conformational Dynamics Probed by Single-Molecule Electron Transfer". Science. 302 (5643): 262–266. Bibcode:2003Sci...302..262Y. doi:10.1126/science.1086911. PMID 14551431. S2CID 18706150.
  • Lu, H. Peter; Xun, Luying; Xie, X. Sunney (1998). "Single-Molecule Enzymatic Dynamics". Science. 282 (5395): 1877–1882. Bibcode:1998Sci...282.1877P. doi:10.1126/science.282.5395.1877. PMID 9836635.

Coherent Raman ImagingEdit

  • Ji, Minbiao; Orringer, Daniel A.; Freudiger, Christian W.; Ramkissoon, Shakti; Liu, Xiaohui; Lau, Darryl; Golby, Alexandra J.; Norton, Isaiah; Hayashi, Marika; Agar, Nathalie Y. R.; Young, Geoffrey S.; Spino, Cathie; Santagata, Sandro; Camelo-Piragua, Sandra; Ligon, Keith L.; Sagher, Oren; Xie, X. Sunney (2013). "Rapid, Label-Free Detection of Brain Tumors with Stimulated Raman Scattering Microscopy". Science Translational Medicine. 5 (201): 201. doi:10.1126/scitranslmed.3005954. PMC 3806096. PMID 24005159.
  • Wang, Meng C.; Min, Wei; Freudiger, Christian W.; Ruvkun, Gary; Xie, X. Sunney (2011). "RNAi Screening for Fat Regulatory Genes with SRS Microscopy". Nat Methods. 8 (2): 135–138. doi:10.1038/nmeth.1556. PMC 3061290. PMID 21240281.
  • Saar, Brian G.; Freudiger, Christian W.; Reichman, Jay; Stanley, C. Michael; Holtom, Gary R.; Xie, X. Sunney (2010). "Video-Rate Molecular Imaging in Vivo with Stimulated Raman Scattering". Science. 330 (6009): 1368–1370. Bibcode:2010Sci...330.1368S. doi:10.1126/science.1197236. PMC 3462359. PMID 21127249.
  • Freudiger, Christian W.; Min, Wei; Saar, Brian G.; Lu, Sijia; Holtom, Gary R.; He, Chengwei; Tsai, Jason C.; Kang, Jing X.; Xie, X. Sunney (2008). "Label-Free Biomedical Imaging with High sensitivity by Stimulated Raman Scattering Microscopy". Science. 322 (5909): 1857–1860. Bibcode:2008Sci...322.1857F. doi:10.1126/science.1165758. PMC 3576036. PMID 19095943.
  • Evans, Conor L.; Potma, Eric O.; Puoris'Haag, Mehron; Côté, Daniel; Lin, Charles P.; Xie, X. Sunney (2005). "Chemical imaging of tissue in vivo with video-rate coherent anti-Stokes Raman scattering microscopy". Proc. Natl. Acad. Sci. 102 (46): 16807–16812. Bibcode:2005PNAS..10216807E. doi:10.1073/pnas.0508282102. PMC 1283840. PMID 16263923.
  • Zumbusch, Andreas; Holtom, Gary R.; Xie, X. Sunney (1999). "Vibrational Microscopy Using Coherent Anti-Stokes Raman Scattering". Phys Rev Lett. 82 (20): 4142–4145. Bibcode:1999PhRvL..82.4142Z. doi:10.1103/physrevlett.82.4142.

Single Molecule ImagingEdit

  • Zhou, Yuexin; Wang, Ping; Tian, Feng; Gao, Ge; Huang, Lei; Wei, Wensheng; Xie, X. Sunney (2017). "Painting a specific chromosome with CRISPR/Cas9 for live-cell imaging". Cell Res. 27 (2): 298–301. doi:10.1038/cr.2017.9. PMC 5339855. PMID 28084328.
  • Chong, Shasha; Min, Wei; Xie, X. Sunney (2010). "Ground-State Depletion Microscopy:Detection Sensitivity of Single-Molecule Optical Absorption at Room Temperature". J Phys Chem Lett. 1 (23): 3316–3322. doi:10.1021/jz1014289.
  • Min, Wei; Lu, Sijia; Chong, Shasha; Roy, Rahul; Holtom, Gary R.; Xie, X. Sunney (2009). "Imaging Chromophores with Undetectable Fluorescence by Stimulated Emission Microscopy". Nature. 461 (7267): 1105–1109. Bibcode:2009Natur.461.1105M. doi:10.1038/nature08438. PMID 19847261. S2CID 1460208.
  • Sánchez, Erik J.; Novotny, Lukas; Xie, X. Sunney (1999). "Near-field Fluorescence Microscopy Based on Two-photon Excitation with Metal Tips". Phys Rev Lett. 82 (20): 4014–4017. Bibcode:1999PhRvL..82.4014S. doi:10.1103/physrevlett.82.4014.
  • Sánchez, Erik J.; Novotny, Lukas; Holtom, G.R.; Xie, X. Sunney (1997). "Room-Temperature Fluorescence Imaging and Spectroscopy of Single Molecules by Two-Photon Excitation". J Phys Chem A. 101 (38): 7019–7023. Bibcode:1997JPCA..101.7019S. doi:10.1021/jp971915b.
  • Xie, X. Sunney; Dunn, Robert C. (1994). "Probing Single Molecule Dynamics". Science. 265 (5170): 361–364. Bibcode:1994Sci...265..361X. doi:10.1126/science.265.5170.361. PMID 17838036. S2CID 6161198.

See alsoEdit


  1. ^ "Archived copy". Archived from the original on 2018-10-10. Retrieved 2018-10-10.{{cite web}}: CS1 maint: archived copy as title (link)
  2. ^ Guinnessy, Paul (2013). "Sunney Xie to receive Leibinger Research Prize for laser technology". Physics Today. doi:10.1063/pt.4.1557. Archived from the original on 2017-08-08. Retrieved 2017-08-08.
  3. ^ "PKU Professor Xiaoliang Sunney Xie awarded Qiu Shi Outstanding Scientist Award of 2017". Peking University. Archived from the original on 2018-10-10. Retrieved 2018-10-10.
  4. ^ "关于公布2017年中国科学院院士增选当选院士名单的公告" (in Chinese). Chinese Academy of Sciences. 2017-11-28. Archived from the original on 2019-11-23. Retrieved 2019-11-22.
  5. ^ "National Academy of Medicine Elects 79 New Members". Archived from the original on 2019-10-18. Retrieved 2016-11-02.
  6. ^ "Albany Medical Center Prize". Archived from the original on 2015-04-23. Retrieved 2015-04-20.
  7. ^ "Peter Debye Award". Archived from the original on 2017-08-25. Retrieved 2015-04-20.
  8. ^ "NIH Director's Pioneer Award 2013". Archived from the original on 2014-02-21. Retrieved 2013-11-14.
  9. ^ "Ellis R. Lippincott Award". Archived from the original on 2016-10-31. Retrieved 2016-11-02.
  10. ^ "Berthold Leibinger Zukunftspreis". Archived from the original on 2016-10-27. Retrieved 2015-04-20.
  11. ^ "Biography of Xie from the Website of the Willis E. Lamb Award (displayed June 4, 2008)". Archived from the original on August 20, 2008. Retrieved June 4, 2008.
  12. ^ "NIH Director's Pioneer Award 2004". Archived from the original on 2016-12-20. Retrieved 2015-04-20.

External linksEdit

  • Homepage of the Xie Group at Harvard