Zalcitabine

Summary

Zalcitabine (2′-3′-dideoxycytidine, ddC), also called dideoxycytidine, is a nucleoside analog reverse-transcriptase inhibitor (NRTI) sold under the trade name Hivid. Zalcitabine was the third antiretroviral to be approved by the Food and Drug Administration (FDA) for the treatment of HIV/AIDS. It is used as part of a combination regimen.

Zalcitabine
Zalcitabine.svg
Clinical data
Trade namesHivid (discontinued)
AHFS/Drugs.comMonograph
Pregnancy
category
  • AU: D
Routes of
administration
Oral
ATC code
Legal status
Legal status
  • UK: POM (Prescription only)
  • US: ℞-only
  • In general: ℞ (Prescription only)
Pharmacokinetic data
Bioavailability>80%
Protein binding<4%
MetabolismHepatic
Elimination half-life2 hours
ExcretionRenal (circa 80%)
Identifiers
  • 4-amino-1-((2R,5S)-5-(hydroxymethyl)tetrahydrofuran-2-yl)pyrimidin-2(1H)-one
CAS Number
  • 7481-89-2 checkY
PubChem CID
  • 24066
IUPHAR/BPS
  • 4828
DrugBank
  • DB00943 checkY
ChemSpider
  • 22498 checkY
UNII
  • 6L3XT8CB3I
KEGG
  • D00412 checkY
ChEBI
  • CHEBI:10101 checkY
ChEMBL
  • ChEMBL853 checkY
NIAID ChemDB
  • 000006
CompTox Dashboard (EPA)
  • DTXSID0023747 Edit this at Wikidata
ECHA InfoCard100.149.677 Edit this at Wikidata
Chemical and physical data
FormulaC9H13N3O3
Molar mass211.221 g·mol−1
3D model (JSmol)
  • Interactive image
  • O=C1/N=C(/N)\C=C/N1[C@@H]2O[C@@H](CC2)CO
  • InChI=1S/C9H13N3O3/c10-7-3-4-12(9(14)11-7)8-2-1-6(5-13)15-8/h3-4,6,8,13H,1-2,5H2,(H2,10,11,14)/t6-,8+/m0/s1 checkY
  • Key:WREGKURFCTUGRC-POYBYMJQSA-N checkY
  (verify)

Zalcitabine appears less potent than some other nucleoside RTIs, has an inconvenient three-times daily frequency and is associated with serious adverse events. For these reasons it is now rarely used to treat human immunodeficiency virus (HIV), and it has even been removed from pharmacies entirely in some countries.[1]

HistoryEdit

Zalcitabine was first synthesized in the 1960s by Jerome Horwitz[2][3] and subsequently developed as an anti-HIV agent by Samuel Broder, Hiroaki Mitsuya, and Robert Yarchoan at the National Cancer Institute (NCI). Like didanosine, it was then licensed because the NCI may not market or sell drugs. The National Institutes of Health (NIH) thus licensed it to Hoffmann-La Roche.

Zalcitabine was the third antiretroviral to be approved by the Food and Drug Administration (FDA) for the treatment of HIV/AIDS. It was approved on June 19, 1992, as a monotherapy and again in 1996 for use in combination with zidovudine (AZT). Using combinations of NRTIs was in practice prior to the second FDA approval and the triple drug combinations with dual NRTIs and a protease inhibitor (PI) were not far off by this time.

The sale and distribution of zalcitabine has been discontinued since December 31, 2006.[4]

Mechanism of actionEdit

Zalcitabine is an analog of pyrimidine. It is a derivative of the naturally existing deoxycytidine, made by replacing the hydroxyl group in position 3' with a hydrogen.

It is phosphorylated in T cells and other HIV target cells into its active triphosphate form, ddCTP. This active metabolite works as a substrate for HIV reverse transcriptase, and also by incorporation into the viral DNA, hence terminating the chain elongation due to the missing hydroxyl group. Since zalcitabine is a reverse transcriptase inhibitor it possesses activity only against retroviruses.

PharmacokineticsEdit

Zalcitabine has a very high oral absorption rate of over 80%. It is predominantly eliminated by the renal route, with a half-life of 2 hours.[5]

Drug interactionsEdit

Lamivudine (3TC) significantly inhibits the intracellular phosphorylation of zalcitabine to the active form, and accordingly the drugs should not be administered together.[5]

Additionally, zalcitabine should not be used with other drugs that can cause peripheral neuropathy, such as didanosine and stavudine.[5]

Adverse eventsEdit

The most common adverse events at the beginning of treatment are nausea and headache. More serious adverse events are peripheral neuropathy, which can occur in up to 33% of patients with advanced disease, oral ulcers, oesophageal ulcers and, rarely, pancreatitis.[5]

ResistanceEdit

Resistance to zalcitabine develops infrequently compared with other nRTIs, and generally only occurs at a low level.[6] The most common mutation observed in vivo is T69D, which does not appear to give rise to cross-resistance to other nRTIs; mutations at positions 65, 74, 75, 184 and 215 in the pol gene are observed more rarely.[5][6]

Specialty drugsEdit

In 1992 dideoxycytidine was listed as a specialty drug.[7]

SourcesEdit

  1. ^ "zalcitabine (CHEBI:10101)". www.ebi.ac.uk. Retrieved 2021-01-18.
  2. ^ Horwitz JR, Chua J, Da Rooge MA, Noel M, Klundt IL (January 1966). "Nucleosides. IX. The formation of 2',2'-unsaturated pyrimidine nucleosides via a novel beta-elimination reaction". The Journal of Organic Chemistry. American Chemical Society (ACS). 31 (1): 205–11. doi:10.1021/jo01339a045. PMID 5900814.
  3. ^ Oral account of the history of AZT, d4T and ddC by Jerome Horwitz and Hiroaki Mitsuya in the documentary film I am alive today - History of an AIDS drug.
  4. ^ "HIVID (zalcitabine) tablets" (PDF). M.D./alert. U.S. Food and Drug Administration. June 2006. Archived from the original (PDF) on 3 February 2012.
  5. ^ a b c d e "HIVID (zalcitabine) tablets. Product information" (PDF). Roche. September 2002. Archived from the original (PDF) on 16 September 2009.
  6. ^ a b Moyle GJ (August 1996). "Use of viral resistance patterns to antiretroviral drugs in optimising selection of drug combinations and sequences". Drugs. Springer Nature. 52 (2): 168–85. doi:10.2165/00003495-199652020-00002. PMID 8841736. S2CID 27709969.
  7. ^ Töglhofer W (1992). "[New, in Austria registered specialty drugs. Hivid (2',3'-dideoxycytidine; ddC)]". Wiener Klinische Wochenschrift. 104 (12): 363–7. PMID 1353278.

Further readingEdit

  • Yarchoan R, Mitsuya H, Broder S (October 1988). "AIDS therapies". Scientific American. Springer Nature. 259 (4): 110–9. Bibcode:1988SciAm.259d.110Y. doi:10.1038/scientificamerican1088-110. PMID 3072667.
  • Remington JP (1990). Gennard AR (ed.). Remington's Pharmaceutical Sciences (18th ed.). Easton, PA: Mack Publishing Company. ISBN 978-0-912734-04-0. OCLC 24381485.
  • Rang HP, Dale MM, Ritter JM (1995). Pharmacology (3rd ed.). United Kingdom: Elsevier Churchill Livingstone. ISBN 978-0-443-07560-5. OCLC 903083639.
  • Mitsuya H, Broder S (March 1986). "Inhibition of the in vitro infectivity and cytopathic effect of human T-lymphotrophic virus type III/lymphadenopathy-associated virus (HTLV-III/LAV) by 2',3'-dideoxynucleosides". Proceedings of the National Academy of Sciences of the United States of America. 83 (6): 1911–5. Bibcode:1986PNAS...83.1911M. doi:10.1073/pnas.83.6.1911. PMC 323194. PMID 3006077.
  • Yarchoan R, Perno CF, Thomas RV, Klecker RW, Allain JP, Wills RJ, et al. (January 1988). "Phase I studies of 2',3'-dideoxycytidine in severe human immunodeficiency virus infection as a single agent and alternating with zidovudine (AZT)". Lancet. Elsevier BV. 1 (8577): 76–81. doi:10.1016/s0140-6736(88)90283-8. PMID 2891981. S2CID 35816286.
  • Mitsuya H, Yarchoan R, Broder S (September 1990). "Molecular targets for AIDS therapy". Science. American Association for the Advancement of Science (AAAS). 249 (4976): 1533–44. Bibcode:1990Sci...249.1533M. doi:10.1126/science.1699273. PMID 1699273.
  • Moyle G (March 1998). "A re-evaluation of zalcitabine". Expert Opinion on Investigational Drugs. Informa Healthcare. 7 (3): 451–62. doi:10.1517/13543784.7.3.451. PMID 15991985.
  • "In Their Own Words: Samuel Broder, M.D." Office of History, National Institutes of Health. 1997-02-02. Retrieved 2018-10-18.
  • "In Their Own Words: Robert Yarchoan, M.D." Office of History, National Institutes of Health. 1998-04-03. Retrieved 2018-10-18.