Understanding the differences between lc-ms vs. Hplc sample analysis

One of the most popular compound separation techniques used across different bioanalytical labs is that of liquid chromatography. In this technique, you have an interaction between individual sample molecules and the stationary and mobile phases of the Liquid Chromatography chamber. The liquid chromatography process is either performed on a sheet or within a chamber with solid support and a supporting liquid mobile phase. 


The liquid mobile phase starts flowing down the solid stationary phase. This allows the mobile phase to carry all the sample components together. In the liquid chromatography unit, the interaction between your sample molecules and the chromatographic medium depends on factors such as charge, affinity binding, size, and hydrophobicity. 


An advanced version of the liquid chromatography technique utilizing high pressure for forcing the sample through the entire column length is called high-performance liquid chromatography. This is currently the most extensively employed quantitative analysis procedure for pharmaceutical industries. 


Mechanism of HPLC unit –

HPLC or theHigh-Performance Liquid Chromatography is an advanced version of the Liquid Chromatographic technique. This process is amenable to a wide range of applications, such as food and pharmaceutical analysis. The main advantage of employing the High-Performance Liquid Chromatography technique is that it is easy to analyze the non-volatile compounds which are otherwise difficult to handle with the gas chromatographic unit. 

There are three types of pumping systems involved here in HPLC – 


Syringe Pumps – 

Syringe pumps help in providing a continuous pulseless flow rate. These pumps are relatively reliable, precise, accurate, and exhibit the potential to hold a large capacity. 


Direct gas-pressure system – 

This is both an inexpensive and reliable HPLC pumping system. However, the process involved in assisting solvent change is a bit difficult. 


Reciprocating pumps – 

Reciprocating pumps are an economical solution specifically designed for providing a constant flow and high pressure. However, sometimes, it may result in pulsing.  


Mass Spectroscopy Analysis – 

Mass Spectrometry is a process wherein the atoms or your molecules undergo the process of ionization for facilitating their separation and detection. The separation and detection of these atoms and molecules are accomplished in accordance with their charges and masses. 


What is LC MS?

When the Liquid Chromatography's separation potential is combined with the detection ability of your Mass Spectrometry, you get an LC MS unit. Combining the analytical methods helps improve the accuracy of the quantification, reducing experimental errors of the entire process. 

Working Mechanism of LC MS – 


LC MS technique separates the sample mixture in accordance with both their physical and chemical properties. This is followed by individual component identification within the dimensions of each peak and detecting them with the help of a mass spectrum. The flow rates involved in your LC MS detection is always less than what you have in your HPLC. The reason for low lc ms method detection is ensuring acomplete ionization processc ms method detection is ensuring a complete ionization process, thereby further maintaining the detection sensitivity of your MS detector beyond 200 microlitres per min. Thus, the LC MS columns are much smaller compared to your HPLC columns, thereby facilitating the accommodation of the lower flow rates of your sample and the sample volumes. 



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